Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability

Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability

Most studies using knockout technologies to examine protein function have relied either on shutting off transcription (conventional conditional knockouts with tetracycline-regulated gene expression or gene disruption) or destroying the mature mRNA (RNAi technology). In both cases, the target protein...

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Bibliographic Details
Main Authors: Laura Wood, Daniel G. Booth, Giulia Vargiu, Shinya Ohta, Flavia deLima Alves, Kumiko Samejima, Tatsuo Fukagawa, Juri Rappsilber, William C. Earnshaw
Format: Article
Language:English
Published: The Royal Society 2016-01-01
Series:Open Biology
Subjects:
kinetochore
cenp-t/w
chromosomes
aid system
silac
mass spectrometry
Online Access:https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150230

Internet

https://royalsocietypublishing.org/doi/pdf/10.1098/rsob.150230

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