Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein
Ionizing radiations induce clustered damage sites known to be severely challenging for the cell's repair machinery. In the present study, we have grafted on a biochip four synthetic oligonucleotide duplexes containing either a single 8-oxoguanine, a single 2'-deoxyribose abasic site or two...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AIMS Press
2015-11-01
|
| Series: | AIMS Materials Science |
| Subjects: | |
| Online Access: | http://www.aimspress.com/Materials/article/514/fulltext.html |
| _version_ | 1818175719209959424 |
|---|---|
| author | Muriel Jourdan Julia Pingel Arnaud Buhot Thierry Livache Jean-François Constant |
| author_facet | Muriel Jourdan Julia Pingel Arnaud Buhot Thierry Livache Jean-François Constant |
| author_sort | Muriel Jourdan |
| collection | DOAJ |
| description | Ionizing radiations induce clustered damage sites known to be severely challenging for the cell's repair machinery. In the present study, we have grafted on a biochip four synthetic oligonucleotide duplexes containing either a single 8-oxoguanine, a single 2'-deoxyribose abasic site or two different clustered damages containing these two lesions on opposite strands. Using a SPR imaging-based DNA array, we qualitatively observed the hierarchy of lesion processing by the base excision repair (BER) enzyme Fpg (Formamidopyrimidine (fapy)-DNA glycosylase). We could notably show that Fpg can convert a cluster of lesions into a potentially lethal double strand break (DSB) and demonstrate that SPR imaging is suitable to investigate the incision steps occurring during the repair process. |
| first_indexed | 2024-12-11T20:04:46Z |
| format | Article |
| id | doaj.art-97d7bb012ab142a59267099df63e5c2a |
| institution | Directory Open Access Journal |
| issn | 2372-0484 |
| language | English |
| last_indexed | 2024-12-11T20:04:46Z |
| publishDate | 2015-11-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Materials Science |
| spelling | doaj.art-97d7bb012ab142a59267099df63e5c2a2022-12-22T00:52:26ZengAIMS PressAIMS Materials Science2372-04842015-11-012447348310.3934/matersci.2015.4.473201504473Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg proteinMuriel Jourdan0Julia PingelArnaud Buhot1Thierry Livache2Jean-François Constant3Univ. Grenoble Alpes, UMR 5250, F-38000 Grenoble, France CNRS, DCM UMR 5250, F-38000 Grenoble, FranceUniv. Grenoble Alpes, INAC, SPrAM, F-38000 Grenoble, France CEA, INAC, SPrAM, F-38000 Grenoble, France CNRS, SPrAM, F-38000 Grenoble, FranceUniv. Grenoble Alpes, INAC, SPrAM, F-38000 Grenoble, France CEA, INAC, SPrAM, F-38000 Grenoble, France CNRS, SPrAM, F-38000 Grenoble, FranceUniv. Grenoble Alpes, UMR 5250, F-38000 Grenoble, France CNRS, DCM UMR 5250, F-38000 Grenoble, FranceIonizing radiations induce clustered damage sites known to be severely challenging for the cell's repair machinery. In the present study, we have grafted on a biochip four synthetic oligonucleotide duplexes containing either a single 8-oxoguanine, a single 2'-deoxyribose abasic site or two different clustered damages containing these two lesions on opposite strands. Using a SPR imaging-based DNA array, we qualitatively observed the hierarchy of lesion processing by the base excision repair (BER) enzyme Fpg (Formamidopyrimidine (fapy)-DNA glycosylase). We could notably show that Fpg can convert a cluster of lesions into a potentially lethal double strand break (DSB) and demonstrate that SPR imaging is suitable to investigate the incision steps occurring during the repair process.http://www.aimspress.com/Materials/article/514/fulltext.htmlsurface plasmon resonance imagingDNA double strand breakFpg8-oxoguanineabasic siteDNA cluster lesion |
| spellingShingle | Muriel Jourdan Julia Pingel Arnaud Buhot Thierry Livache Jean-François Constant Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein AIMS Materials Science surface plasmon resonance imaging DNA double strand break Fpg 8-oxoguanine abasic site DNA cluster lesion |
| title | Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein |
| title_full | Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein |
| title_fullStr | Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein |
| title_full_unstemmed | Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein |
| title_short | Surface plasmon resonance imaging of the conversion of clustered DNA lesions into double strand breaks by Fpg protein |
| title_sort | surface plasmon resonance imaging of the conversion of clustered dna lesions into double strand breaks by fpg protein |
| topic | surface plasmon resonance imaging DNA double strand break Fpg 8-oxoguanine abasic site DNA cluster lesion |
| url | http://www.aimspress.com/Materials/article/514/fulltext.html |
| work_keys_str_mv | AT murieljourdan surfaceplasmonresonanceimagingoftheconversionofclustereddnalesionsintodoublestrandbreaksbyfpgprotein AT juliapingel surfaceplasmonresonanceimagingoftheconversionofclustereddnalesionsintodoublestrandbreaksbyfpgprotein AT arnaudbuhot surfaceplasmonresonanceimagingoftheconversionofclustereddnalesionsintodoublestrandbreaksbyfpgprotein AT thierrylivache surfaceplasmonresonanceimagingoftheconversionofclustereddnalesionsintodoublestrandbreaksbyfpgprotein AT jeanfrancoisconstant surfaceplasmonresonanceimagingoftheconversionofclustereddnalesionsintodoublestrandbreaksbyfpgprotein |