Grid batch-dependent tuning of glow discharge parameters

Grid batch-dependent tuning of glow discharge parameters

Sample preparation on cryo-EM grids can give various results, from very thin ice and homogeneous particle distribution (ideal case) to unwanted behavior such as particles around the “holes” or complexes that do not entirely correspond to the one in solution (real life). We recently run into such a c...

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Bibliographic Details
Main Authors: Ramy Kazan, Gabrielle Bourgeois, Dominique Carisetti, Ileana Florea, Eric Larquet, Jean-Luc Maurice, Yves Mechulam, François Ozanam, Emmanuelle Schmitt, Pierre-Damien Coureux
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-08-01
Series:Frontiers in Molecular Biosciences
Subjects:
cryo-EM
sample preparation
glow discharge
quantifoil
continuous carbon
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2022.910218/full

Internet

https://www.frontiersin.org/articles/10.3389/fmolb.2022.910218/full

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