Macromolecular characterization of high β-glucan oat lines
Oat (Avena sativa) is a cereal grain rich in fibers, proteins, vitamins and minerals. Oats have been linked to several health benefits, such as lowering blood cholesterol levels, counteracting cardiovascular disease and regulating blood sugar levels. This study aimed to characterize two new oat line...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024005838 |
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| author | Abhinav Majumdar Aina Belén Gil-González Anna Barjuan Grau Roya R.R. Sardari Olof Larsson Aishwarya Thyagarajan Andreas Hansson Oswaldo Hernández-Hernández Olof Olsson José Alfredo Zambrano |
| author_facet | Abhinav Majumdar Aina Belén Gil-González Anna Barjuan Grau Roya R.R. Sardari Olof Larsson Aishwarya Thyagarajan Andreas Hansson Oswaldo Hernández-Hernández Olof Olsson José Alfredo Zambrano |
| author_sort | Abhinav Majumdar |
| collection | DOAJ |
| description | Oat (Avena sativa) is a cereal grain rich in fibers, proteins, vitamins and minerals. Oats have been linked to several health benefits, such as lowering blood cholesterol levels, counteracting cardiovascular disease and regulating blood sugar levels. This study aimed to characterize two new oat lines with high β-glucan content emanating from ethyl methyl sulphonate mutagenesis on the Lantmännen elite variety Belinda. Two of the mutated lines, and the mother variety Belinda, were profiled for β-glucan, arabinoxylan, total dietary fiber and starch composition. In addition, total lipid and protein content, amino acid composition and β-glucan molecular weights were analyzed. The high levels of β-glucan resulted in a significant increase in total dietary fiber, but no correlation could be established between higher or lower levels of the assayed macromolecules, i.e., between arabinoxylan-, starch-, lipid- or protein levels in the mutated lines compared to the reference. The results indicate separate biosynthetic pathways for β-glucans and other macromolecules and an independent regulation of the different polysaccharides studied. Therefore, ethyl methyl sulphonate mutagenesis can be used to increase levels of multiple macromolecules in the same line. |
| first_indexed | 2024-03-08T06:55:09Z |
| format | Article |
| id | doaj.art-9824c367e48f41d29f7c4b091984097c |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T06:55:09Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-9824c367e48f41d29f7c4b091984097c2024-02-03T06:37:51ZengElsevierHeliyon2405-84402024-01-01102e24552Macromolecular characterization of high β-glucan oat linesAbhinav Majumdar0Aina Belén Gil-González1Anna Barjuan Grau2Roya R.R. Sardari3Olof Larsson4Aishwarya Thyagarajan5Andreas Hansson6Oswaldo Hernández-Hernández7Olof Olsson8José Alfredo Zambrano9Division of Pure and Applied Biochemistry, Lund University, Box 124, SE 22100, Lund, SwedenDivision of Pure and Applied Biochemistry, Lund University, Box 124, SE 22100, Lund, SwedenDivision of Pure and Applied Biochemistry, Lund University, Box 124, SE 22100, Lund, SwedenDepartment of Biotechnology, Lund University, Box 124, SE 22100, Lund, SwedenCropTailorAB, c/o Department of Pure and Applied Biochemistry, Lund University, Box 124, SE 22100, Lund, SwedenDivision of Pure and Applied Biochemistry, Lund University, Box 124, SE 22100, Lund, SwedenCropTailorAB, c/o Department of Pure and Applied Biochemistry, Lund University, Box 124, SE 22100, Lund, SwedenInstitute of Food Science Research (CIAL), CSIC-UAM, c/Nicolás Cabrera, 9, 28049, Madrid, SpainDivision of Pure and Applied Biochemistry, Lund University, Box 124, SE 22100, Lund, SwedenDivision of Pure and Applied Biochemistry, Lund University, Box 124, SE 22100, Lund, Sweden; Corresponding author.Oat (Avena sativa) is a cereal grain rich in fibers, proteins, vitamins and minerals. Oats have been linked to several health benefits, such as lowering blood cholesterol levels, counteracting cardiovascular disease and regulating blood sugar levels. This study aimed to characterize two new oat lines with high β-glucan content emanating from ethyl methyl sulphonate mutagenesis on the Lantmännen elite variety Belinda. Two of the mutated lines, and the mother variety Belinda, were profiled for β-glucan, arabinoxylan, total dietary fiber and starch composition. In addition, total lipid and protein content, amino acid composition and β-glucan molecular weights were analyzed. The high levels of β-glucan resulted in a significant increase in total dietary fiber, but no correlation could be established between higher or lower levels of the assayed macromolecules, i.e., between arabinoxylan-, starch-, lipid- or protein levels in the mutated lines compared to the reference. The results indicate separate biosynthetic pathways for β-glucans and other macromolecules and an independent regulation of the different polysaccharides studied. Therefore, ethyl methyl sulphonate mutagenesis can be used to increase levels of multiple macromolecules in the same line.http://www.sciencedirect.com/science/article/pii/S2405844024005838Avena sativaDietary fibersβ-GlucanMacromolecular quantificationsAmino acid analysisMolecular weight determination |
| spellingShingle | Abhinav Majumdar Aina Belén Gil-González Anna Barjuan Grau Roya R.R. Sardari Olof Larsson Aishwarya Thyagarajan Andreas Hansson Oswaldo Hernández-Hernández Olof Olsson José Alfredo Zambrano Macromolecular characterization of high β-glucan oat lines Heliyon Avena sativa Dietary fibers β-Glucan Macromolecular quantifications Amino acid analysis Molecular weight determination |
| title | Macromolecular characterization of high β-glucan oat lines |
| title_full | Macromolecular characterization of high β-glucan oat lines |
| title_fullStr | Macromolecular characterization of high β-glucan oat lines |
| title_full_unstemmed | Macromolecular characterization of high β-glucan oat lines |
| title_short | Macromolecular characterization of high β-glucan oat lines |
| title_sort | macromolecular characterization of high β glucan oat lines |
| topic | Avena sativa Dietary fibers β-Glucan Macromolecular quantifications Amino acid analysis Molecular weight determination |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024005838 |
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