Evaluation of a direct phage DNA detection-based Taqman qPCR methodology for quantification of phage and its application in rapid ultrasensitive identification of Acinetobacter baumannii

Evaluation of a direct phage DNA detection-based Taqman qPCR methodology for quantification of phage and its application in rapid ultrasensitive identification of Acinetobacter baumannii

Abstract Background Rapid phage enumeration/quantitation and viable bacteria determination is critical for phage application and treatment of infectious patients caused by the pathogenic bacteria. Methods In the current study, a direct phage DNA detection-based Taqman qPCR methodology for quantifica...

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Bibliographic Details
Main Authors: Jun Luo, Min Liu, Peng Wang, Qianyuan Li, Chunhua Luo, Hongping Wei, Yuanyuan Hu, Junping Yu
Format: Article
Language:English
Published: BMC 2022-06-01
Series:BMC Infectious Diseases
Subjects:
Direct phage DNA detection-based Taqman qPCR
Quantification of phage
Viable Acinetobacter baumannii
Detection
Swab/bronchoalveolar lavage fluid
Online Access:https://doi.org/10.1186/s12879-022-07493-1

Internet

https://doi.org/10.1186/s12879-022-07493-1

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