Simulation vs. reality: a comparison of in silico distance predictions with DEER and FRET measurements.

Site specific incorporation of molecular probes such as fluorescent- and nitroxide spin-labels into biomolecules, and subsequent analysis by Förster resonance energy transfer (FRET) and double electron-electron resonance (DEER) can elucidate the distance and distance-changes between the probes. Howe...

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Bibliographic Details
Main Authors: Daniel Klose, Johann P Klare, Dina Grohmann, Christopher W M Kay, Finn Werner, Heinz-Jürgen Steinhoff
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3382601?pdf=render