Double-Labeled Fluorescent Probes for 5′ Nuclease Assays: Purification and Performance Evaluation
An inexpensive method for the purification and evaluation of user-synthesized or crude commercially prepared double-labeled fluorescent probes is presented. These probes exhibit the characteristics required for use in 5′-nuclease assays, including efficient reporter dye quenching, target specificity...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-06-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97226rr02 |