Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform
Riazanski et al describe a platform to capture extracellular vesicles (EVs) using a nanoporous silicon nitride membrane, investigate the expression of NHE1 protein on the surface of EVs and monitor the transport of Na+ and H+ at the single EV level. The authors report a mechanistic function of the p...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-01-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-02965-7 |
| _version_ | 1798026175020269568 |
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| author | Vladimir Riazanski Gerardo Mauleon Kilean Lucas Samuel Walker Adriana M. Zimnicka James L. McGrath Deborah J. Nelson |
| author_facet | Vladimir Riazanski Gerardo Mauleon Kilean Lucas Samuel Walker Adriana M. Zimnicka James L. McGrath Deborah J. Nelson |
| author_sort | Vladimir Riazanski |
| collection | DOAJ |
| description | Riazanski et al describe a platform to capture extracellular vesicles (EVs) using a nanoporous silicon nitride membrane, investigate the expression of NHE1 protein on the surface of EVs and monitor the transport of Na+ and H+ at the single EV level. The authors report a mechanistic function of the proteins found in EVs and specifically identify NHE1 on a single EV, where it maintains pH neutrality within single vesicles. |
| first_indexed | 2024-04-11T18:31:08Z |
| format | Article |
| id | doaj.art-98864c1740fb4e14ba8f46b059eb5d8e |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-04-11T18:31:08Z |
| publishDate | 2022-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-98864c1740fb4e14ba8f46b059eb5d8e2022-12-22T04:09:26ZengNature PortfolioCommunications Biology2399-36422022-01-015111310.1038/s42003-021-02965-7Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platformVladimir Riazanski0Gerardo Mauleon1Kilean Lucas2Samuel Walker3Adriana M. Zimnicka4James L. McGrath5Deborah J. Nelson6Department of Pharmacological and Physiological Sciences, The University of ChicagoDepartment of Pharmacological and Physiological Sciences, The University of ChicagoDepartment of Biomedical Engineering, University of RochesterDepartment of Biomedical Engineering, University of RochesterDepartment of Pharmacological and Physiological Sciences, The University of ChicagoDepartment of Biomedical Engineering, University of RochesterDepartment of Pharmacological and Physiological Sciences, The University of ChicagoRiazanski et al describe a platform to capture extracellular vesicles (EVs) using a nanoporous silicon nitride membrane, investigate the expression of NHE1 protein on the surface of EVs and monitor the transport of Na+ and H+ at the single EV level. The authors report a mechanistic function of the proteins found in EVs and specifically identify NHE1 on a single EV, where it maintains pH neutrality within single vesicles.https://doi.org/10.1038/s42003-021-02965-7 |
| spellingShingle | Vladimir Riazanski Gerardo Mauleon Kilean Lucas Samuel Walker Adriana M. Zimnicka James L. McGrath Deborah J. Nelson Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform Communications Biology |
| title | Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform |
| title_full | Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform |
| title_fullStr | Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform |
| title_full_unstemmed | Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform |
| title_short | Real time imaging of single extracellular vesicle pH regulation in a microfluidic cross-flow filtration platform |
| title_sort | real time imaging of single extracellular vesicle ph regulation in a microfluidic cross flow filtration platform |
| url | https://doi.org/10.1038/s42003-021-02965-7 |
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