CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus

CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus

Abstract Background The CRISPR/dCas9 system is a powerful tool to activate the transcription of target genes in eukaryotic or prokaryotic cells, but lacks assays in complex conditions, such as the biosynthesis of secondary metabolites. Results In this study, to improve the transcription of the heter...

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Bibliographic Details
Main Authors: Ran Peng, Ye Wang, Wan-wan Feng, Xin-jing Yue, Jiang-he Chen, Xiao-zhuang Hu, Zhi-feng Li, Duo-hong Sheng, You-ming Zhang, Yue-zhong Li
Format: Article
Language:English
Published: BMC 2018-01-01
Series:Microbial Cell Factories
Subjects:
CRISPR/dCas9 activation
sgRNAs
Activator proteins
Promoter
Transcriptional improvement
Biosynthetic gene cluster
Online Access:http://link.springer.com/article/10.1186/s12934-018-0867-1

Internet

http://link.springer.com/article/10.1186/s12934-018-0867-1

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