Rapid generation of human recombinant monoclonal antibodies from antibody-secreting cells using ferrofluid-based technology

Rapid generation of human recombinant monoclonal antibodies from antibody-secreting cells using ferrofluid-based technology

Monoclonal antibodies (mAbs) are one of the most important classes of biologics with high therapeutic and diagnostic value, but traditional methods for mAbs generation, such as hybridoma screening and phage display, have limitations, including low efficiency and loss of natural chain pairing. To ove...

Full description

Bibliographic Details
Main Authors: Veronica Strazza, Marco Rossi, Andrea Avati, Giusy Tiseo, Marco Falcone, Maria Grazia Cusi, Francesco Menichetti, Paola Ricciardi-Castagnoli, Cristina Tinti, Piero Pileri
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-04-01
Series:Frontiers in Immunology
Subjects:
human monoclonal antibodies
antibody secreting cells (ASCs)
single cell PCR
CD138-ferrofluid
transcriptionally active PCR (TAP)
antigen-specific ASCs
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2024.1341389/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2024.1341389/full

Similar Items