Characterization of Cold-Tolerant <i>Saccharomyces cerevisiae</i> Cheongdo Using Phenotype Microarray
The cold-tolerant yeast <i>Saccharomyces cerevisiae</i> is industrially useful for lager fermentation, high-quality wine, and frozen dough production. <i>S. cerevisiae</i> Cheongdo is a recent isolate from frozen peach samples which has a good fermentation performance at low...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/5/982 |
| _version_ | 1797535575949967360 |
|---|---|
| author | Kyung-Mi Jung Jongbeom Park Jueun Jang Seok-Hwa Jung Sang Han Lee Soo Rin Kim |
| author_facet | Kyung-Mi Jung Jongbeom Park Jueun Jang Seok-Hwa Jung Sang Han Lee Soo Rin Kim |
| author_sort | Kyung-Mi Jung |
| collection | DOAJ |
| description | The cold-tolerant yeast <i>Saccharomyces cerevisiae</i> is industrially useful for lager fermentation, high-quality wine, and frozen dough production. <i>S. cerevisiae</i> Cheongdo is a recent isolate from frozen peach samples which has a good fermentation performance at low temperatures and desirable flavor profiles. Here, phenotype microarray was used to investigate industrial potentials of <i>S. cerevisiae</i> Cheongdo using 192 carbon sources. Compared to commercial wine yeast <i>S. cerevisiae</i> EC1118, Cheongdo showed significantly different growth rates on 34 substrates. The principal component analysis of the results highlighted that the better growth of Cheongdo on galactose than on EC1118 was the most significant difference between the two strains. The intact <i>GAL4</i> gene and the galactose fermentation performance at a low temperatures suggested that <i>S. cerevisiae</i> Cheongdo is a promising host for industrial fermentation rich in galactose, such as lactose and agarose. |
| first_indexed | 2024-03-10T11:46:25Z |
| format | Article |
| id | doaj.art-98ddea2de01c4d1186e6e5cec1c24e04 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:46:25Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-98ddea2de01c4d1186e6e5cec1c24e042023-11-21T18:03:41ZengMDPI AGMicroorganisms2076-26072021-04-019598210.3390/microorganisms9050982Characterization of Cold-Tolerant <i>Saccharomyces cerevisiae</i> Cheongdo Using Phenotype MicroarrayKyung-Mi Jung0Jongbeom Park1Jueun Jang2Seok-Hwa Jung3Sang Han Lee4Soo Rin Kim5Cheongdo Peach Research Institute, Gyeongsangbuk-Do Agricultural Technology Administration, Cheongdo 38315, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaThe cold-tolerant yeast <i>Saccharomyces cerevisiae</i> is industrially useful for lager fermentation, high-quality wine, and frozen dough production. <i>S. cerevisiae</i> Cheongdo is a recent isolate from frozen peach samples which has a good fermentation performance at low temperatures and desirable flavor profiles. Here, phenotype microarray was used to investigate industrial potentials of <i>S. cerevisiae</i> Cheongdo using 192 carbon sources. Compared to commercial wine yeast <i>S. cerevisiae</i> EC1118, Cheongdo showed significantly different growth rates on 34 substrates. The principal component analysis of the results highlighted that the better growth of Cheongdo on galactose than on EC1118 was the most significant difference between the two strains. The intact <i>GAL4</i> gene and the galactose fermentation performance at a low temperatures suggested that <i>S. cerevisiae</i> Cheongdo is a promising host for industrial fermentation rich in galactose, such as lactose and agarose.https://www.mdpi.com/2076-2607/9/5/982phenotype microarrayyeastcold tolerancegalactoseGAL4 |
| spellingShingle | Kyung-Mi Jung Jongbeom Park Jueun Jang Seok-Hwa Jung Sang Han Lee Soo Rin Kim Characterization of Cold-Tolerant <i>Saccharomyces cerevisiae</i> Cheongdo Using Phenotype Microarray Microorganisms phenotype microarray yeast cold tolerance galactose GAL4 |
| title | Characterization of Cold-Tolerant <i>Saccharomyces cerevisiae</i> Cheongdo Using Phenotype Microarray |
| title_full | Characterization of Cold-Tolerant <i>Saccharomyces cerevisiae</i> Cheongdo Using Phenotype Microarray |
| title_fullStr | Characterization of Cold-Tolerant <i>Saccharomyces cerevisiae</i> Cheongdo Using Phenotype Microarray |
| title_full_unstemmed | Characterization of Cold-Tolerant <i>Saccharomyces cerevisiae</i> Cheongdo Using Phenotype Microarray |
| title_short | Characterization of Cold-Tolerant <i>Saccharomyces cerevisiae</i> Cheongdo Using Phenotype Microarray |
| title_sort | characterization of cold tolerant i saccharomyces cerevisiae i cheongdo using phenotype microarray |
| topic | phenotype microarray yeast cold tolerance galactose GAL4 |
| url | https://www.mdpi.com/2076-2607/9/5/982 |
| work_keys_str_mv | AT kyungmijung characterizationofcoldtolerantisaccharomycescerevisiaeicheongdousingphenotypemicroarray AT jongbeompark characterizationofcoldtolerantisaccharomycescerevisiaeicheongdousingphenotypemicroarray AT jueunjang characterizationofcoldtolerantisaccharomycescerevisiaeicheongdousingphenotypemicroarray AT seokhwajung characterizationofcoldtolerantisaccharomycescerevisiaeicheongdousingphenotypemicroarray AT sanghanlee characterizationofcoldtolerantisaccharomycescerevisiaeicheongdousingphenotypemicroarray AT soorinkim characterizationofcoldtolerantisaccharomycescerevisiaeicheongdousingphenotypemicroarray |