Purification and characterization of an antioxidant peptide (GSQ) from Chinese leek (Allium tuberosum Rottler) seeds
A peptide with antioxidant activity was purified from Chinese leek seeds by successive chromatographic methods. The sequence was determined as Gly-Ser-Gln (290.10 Da) by LC-MS/MS. This purified peptide, discovered for the first time from Chinese leek seeds and designated as CLP-2, could efficiently...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-09-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464614001856 |
| _version_ | 1818958093875150848 |
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| author | Jing Hong Tao-Tao Chen Pei Hu Jie Yang Shao-Yun Wang |
| author_facet | Jing Hong Tao-Tao Chen Pei Hu Jie Yang Shao-Yun Wang |
| author_sort | Jing Hong |
| collection | DOAJ |
| description | A peptide with antioxidant activity was purified from Chinese leek seeds by successive chromatographic methods. The sequence was determined as Gly-Ser-Gln (290.10 Da) by LC-MS/MS. This purified peptide, discovered for the first time from Chinese leek seeds and designated as CLP-2, could efficiently quench free radicals and exhibit moderate reducing power. The concentrations for 50% activities for its radical scavenging effect against DPPH, ABTS+ and O2− were 0.61, 0.04 and 0.70 mg/mL, respectively. In addition, the peptide protected LO2 cells against hydrogen peroxide-induced damage at a concentration of 10 µg/mL. Furthermore, CLP-2 inhibited growth of Gram-negative and Gram-positive bacteria. |
| first_indexed | 2024-12-20T11:20:16Z |
| format | Article |
| id | doaj.art-98f15ff98345414ea89486bef170ccb2 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T11:20:16Z |
| publishDate | 2014-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-98f15ff98345414ea89486bef170ccb22022-12-21T19:42:32ZengElsevierJournal of Functional Foods1756-46462014-09-0110144153Purification and characterization of an antioxidant peptide (GSQ) from Chinese leek (Allium tuberosum Rottler) seedsJing Hong0Tao-Tao Chen1Pei Hu2Jie Yang3Shao-Yun Wang4College of Biological Science and Technology, Fuzhou University, 2 Xue Yuan Road, University Town, Fuzhou, Fujian 350108, ChinaCollege of Biological Science and Technology, Fuzhou University, 2 Xue Yuan Road, University Town, Fuzhou, Fujian 350108, ChinaCollege of Biological Science and Technology, Fuzhou University, 2 Xue Yuan Road, University Town, Fuzhou, Fujian 350108, ChinaCollege of Biological Science and Technology, Fuzhou University, 2 Xue Yuan Road, University Town, Fuzhou, Fujian 350108, ChinaCorresponding author. Tel.: +86 591 2286 6375; fax: +86 591 2286 6278.; College of Biological Science and Technology, Fuzhou University, 2 Xue Yuan Road, University Town, Fuzhou, Fujian 350108, ChinaA peptide with antioxidant activity was purified from Chinese leek seeds by successive chromatographic methods. The sequence was determined as Gly-Ser-Gln (290.10 Da) by LC-MS/MS. This purified peptide, discovered for the first time from Chinese leek seeds and designated as CLP-2, could efficiently quench free radicals and exhibit moderate reducing power. The concentrations for 50% activities for its radical scavenging effect against DPPH, ABTS+ and O2− were 0.61, 0.04 and 0.70 mg/mL, respectively. In addition, the peptide protected LO2 cells against hydrogen peroxide-induced damage at a concentration of 10 µg/mL. Furthermore, CLP-2 inhibited growth of Gram-negative and Gram-positive bacteria.http://www.sciencedirect.com/science/article/pii/S1756464614001856Chinese leek seedsTripeptideAntioxidantPurification |
| spellingShingle | Jing Hong Tao-Tao Chen Pei Hu Jie Yang Shao-Yun Wang Purification and characterization of an antioxidant peptide (GSQ) from Chinese leek (Allium tuberosum Rottler) seeds Journal of Functional Foods Chinese leek seeds Tripeptide Antioxidant Purification |
| title | Purification and characterization of an antioxidant peptide (GSQ) from Chinese leek (Allium tuberosum Rottler) seeds |
| title_full | Purification and characterization of an antioxidant peptide (GSQ) from Chinese leek (Allium tuberosum Rottler) seeds |
| title_fullStr | Purification and characterization of an antioxidant peptide (GSQ) from Chinese leek (Allium tuberosum Rottler) seeds |
| title_full_unstemmed | Purification and characterization of an antioxidant peptide (GSQ) from Chinese leek (Allium tuberosum Rottler) seeds |
| title_short | Purification and characterization of an antioxidant peptide (GSQ) from Chinese leek (Allium tuberosum Rottler) seeds |
| title_sort | purification and characterization of an antioxidant peptide gsq from chinese leek allium tuberosum rottler seeds |
| topic | Chinese leek seeds Tripeptide Antioxidant Purification |
| url | http://www.sciencedirect.com/science/article/pii/S1756464614001856 |
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