Acid Extraction of Total Histone from Colon Cancer HCT116 Cells
Histone acid extraction assay is a popular method to determine histone modification levels in mammalian cells. It includes three steps: first, histones are released from chromatin by sulfuric acid; trichloroacetate (TCA) is then added to precipitate histones; and finally, histones are dissolved in d...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2016-11-01
|
| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=2023&type=0 |