Acid Extraction of Total Histone from Colon Cancer HCT116 Cells
Histone acid extraction assay is a popular method to determine histone modification levels in mammalian cells. It includes three steps: first, histones are released from chromatin by sulfuric acid; trichloroacetate (TCA) is then added to precipitate histones; and finally, histones are dissolved in d...
| Hlavní autoři: | , |
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| Médium: | Článek |
| Jazyk: | English |
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Bio-protocol LLC
2016-11-01
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| Edice: | Bio-Protocol |
| On-line přístup: | https://bio-protocol.org/en/bpdetail?id=2023&type=0 |