New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças
Cachaça is a popular spirit produced in Brazil, obtained by distillation of fermented sugar cane. Among the contaminants arising from production, ethyl carbamate is a carcinogenic compound that occurs naturally in fermented foods and beverages; in Brazil, the maximum limit established by current leg...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fnut.2018.00021/full |
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| author | Tatiane Melina Guerreiro Kumi Shiota Ozawa Estela de Oliveira Lima Carlos Fernando Odir Rodrigues Melo Diogo Noin de Oliveira Simone Pereira do Nascimento Triano Rodrigo Ramos Catharino |
| author_facet | Tatiane Melina Guerreiro Kumi Shiota Ozawa Estela de Oliveira Lima Carlos Fernando Odir Rodrigues Melo Diogo Noin de Oliveira Simone Pereira do Nascimento Triano Rodrigo Ramos Catharino |
| author_sort | Tatiane Melina Guerreiro |
| collection | DOAJ |
| description | Cachaça is a popular spirit produced in Brazil, obtained by distillation of fermented sugar cane. Among the contaminants arising from production, ethyl carbamate is a carcinogenic compound that occurs naturally in fermented foods and beverages; in Brazil, the maximum limit established by current legislation is 150 µg L−1. Quality control is usually performed using gas chromatography; however, robustness and reproducibility of quantitative results may be severely impaired, as the addition of 6–30 g L−1 of sucrose is a common procedure for taste standardization, directly interfering in the results. This work describes the development of a novel method to improve ethyl carbamate quantification in cachaças using a new approach of QuEChERS extraction based on salting-out phenomenon, to effectively separate ethanol from sugar-containing water. Eighteen different brands of cachaça were analyzed. The proposed methodology was able to eliminate components that contaminate the sample flow path in the gas chromatography system, while improving precision and accuracy by using a triple-quadrupole approach, in comparison with the methodology usually employed: direct analysis of cachaça samples with no sample prep. Results indicate that this approach is more effective due to the removal of sugar content, with no impact in costs per analysis. |
| first_indexed | 2024-12-21T21:02:10Z |
| format | Article |
| id | doaj.art-9902dbfd51034e97a31141b234866a6b |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-21T21:02:10Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-9902dbfd51034e97a31141b234866a6b2022-12-21T18:50:25ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2018-04-01510.3389/fnut.2018.00021326617New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaçasTatiane Melina Guerreiro0Kumi Shiota Ozawa1Estela de Oliveira Lima2Carlos Fernando Odir Rodrigues Melo3Diogo Noin de Oliveira4Simone Pereira do Nascimento Triano5Rodrigo Ramos Catharino6Innovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, BrazilAgilent Technologies (Brazil), São Paulo, BrazilInnovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, BrazilInnovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, BrazilInnovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, BrazilAgilent Technologies (Brazil), São Paulo, BrazilInnovare Biomarkers Laboratory, School of Pharmaceutical Sciences, University of Campinas, Campinas, BrazilCachaça is a popular spirit produced in Brazil, obtained by distillation of fermented sugar cane. Among the contaminants arising from production, ethyl carbamate is a carcinogenic compound that occurs naturally in fermented foods and beverages; in Brazil, the maximum limit established by current legislation is 150 µg L−1. Quality control is usually performed using gas chromatography; however, robustness and reproducibility of quantitative results may be severely impaired, as the addition of 6–30 g L−1 of sucrose is a common procedure for taste standardization, directly interfering in the results. This work describes the development of a novel method to improve ethyl carbamate quantification in cachaças using a new approach of QuEChERS extraction based on salting-out phenomenon, to effectively separate ethanol from sugar-containing water. Eighteen different brands of cachaça were analyzed. The proposed methodology was able to eliminate components that contaminate the sample flow path in the gas chromatography system, while improving precision and accuracy by using a triple-quadrupole approach, in comparison with the methodology usually employed: direct analysis of cachaça samples with no sample prep. Results indicate that this approach is more effective due to the removal of sugar content, with no impact in costs per analysis.http://journal.frontiersin.org/article/10.3389/fnut.2018.00021/fullalcoholic beveragescachaçaethyl carbamategas chromatography–mass spectrometryQuEChERS |
| spellingShingle | Tatiane Melina Guerreiro Kumi Shiota Ozawa Estela de Oliveira Lima Carlos Fernando Odir Rodrigues Melo Diogo Noin de Oliveira Simone Pereira do Nascimento Triano Rodrigo Ramos Catharino New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças Frontiers in Nutrition alcoholic beverages cachaça ethyl carbamate gas chromatography–mass spectrometry QuEChERS |
| title | New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças |
| title_full | New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças |
| title_fullStr | New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças |
| title_full_unstemmed | New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças |
| title_short | New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachaças |
| title_sort | new approach of quechers and gc ms triple quadrupole for the determination of ethyl carbamate content in brazilian cachacas |
| topic | alcoholic beverages cachaça ethyl carbamate gas chromatography–mass spectrometry QuEChERS |
| url | http://journal.frontiersin.org/article/10.3389/fnut.2018.00021/full |
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