A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol

Abstract Background In the past few years, there has been an explosion in single-cell transcriptomics datasets, yet in vivo confirmation of these datasets is hampered in plants due to lack of robust validation methods. Likewise, modeling of plant development is hampered by paucity of spatial gene ex...

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Bibliographic Details
Main Authors: Tian Huang, Bruno Guillotin, Ramin Rahni, Kenneth D. Birnbaum, Doris Wagner
Format: Article
Language:English
Published: BMC 2023-11-01
Series:Plant Methods
Subjects:
Online Access:https://doi.org/10.1186/s13007-023-01108-9