A rapid and sensitive, multiplex, whole mount RNA fluorescence in situ hybridization and immunohistochemistry protocol
Abstract Background In the past few years, there has been an explosion in single-cell transcriptomics datasets, yet in vivo confirmation of these datasets is hampered in plants due to lack of robust validation methods. Likewise, modeling of plant development is hampered by paucity of spatial gene ex...
Main Authors: | Tian Huang, Bruno Guillotin, Ramin Rahni, Kenneth D. Birnbaum, Doris Wagner |
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Format: | Article |
Language: | English |
Published: |
BMC
2023-11-01
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Series: | Plant Methods |
Subjects: | |
Online Access: | https://doi.org/10.1186/s13007-023-01108-9 |
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