Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis

Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December 2019 and quickly spread around the world, forcing global health authorities to develop protocols for its diagnosis. Here we report dimer formation in the N2 primers–probe set (CDC 2019-nCoV Real-Time RT-PCR) used in the...

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Bibliographic Details
Main Authors: Lauren Hubert Jaeger, Thiago César Nascimento, Fabíola Dutra Rocha, Fernanda Maria Pinto Vilela, Ana Paula do Nascimento Duque, Lívia Mara Silva, Lorena Rodrigues Riani, João Paulo Moreira, Jéssica Mara de Assis Chagas, Thamiris Vilela Pereira, Carmen Gomide Pinto Perches, Aripuana Sakurada Aranha Watanabe, Lyderson Facio Viccini, Marcelo Silva Silvério, José Otávio do Amaral Corrêa, Olavo dos Santos Pereira-Junior, Frederico Pittella
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:International Journal of Infectious Diseases
Subjects:
Coronavirus
Diagnostic techniques and procedures
COVID-19
RT-qPCR
Online Access:http://www.sciencedirect.com/science/article/pii/S1201971220322839

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http://www.sciencedirect.com/science/article/pii/S1201971220322839

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