Generation of precise calibration curve for absolute quantification of DNA target by Real-Time PCR. A method for accurate molecular monitoring of t(14;18)-bearing cells in Follicular Lymphoma

Generation of precise calibration curve for absolute quantification of DNA target by Real-Time PCR. A method for accurate molecular monitoring of t(14;18)-bearing cells in Follicular Lymphoma

Objective: Determination of absolute DNA concentration by Real-Time PCR is mandatory for an accurate molecular diagnosis. The critical step is a precise and reliable calibration curve. Plasmid DNA containing cloned target sequences is widely used as standards in quantitative PCR. Usually, the initia...

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Bibliographic Details
Main Authors: R. Di Francia, R. De Filippi, S. Crisci, G. Iaccarino, O. Catapano, E. Varriale, F. Frigeri, A. Pinto
Format: Article
Language:English
Published: Verduci Editore 2021-05-01
Series:World Cancer Research Journal
Subjects:
molecular diagnostics
standard curve
competitive-pcr
t(14;18)bcl2/igh major breakpoint region
Online Access:https://www.wcrj.net/wp-content/uploads/sites/5/2022/03/e1969.pdf

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https://www.wcrj.net/wp-content/uploads/sites/5/2022/03/e1969.pdf

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