A Rapid Method for the Identification of Fresh and Processed <i>Pagellus erythrinus</i> Species against Frauds
The commercialization of porgies or seabreams of the family <i>Sparidae</i> has greatly increased in the last decade, and some valuable species have become subject to seafood substitution. DNA regions currently used for fish species identification in fresh and processed products belong t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/10/1397 |
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| author | Marina Ceruso Celestina Mascolo Pasquale De Luca Iolanda Venuti Giorgio Smaldone Elio Biffali Aniello Anastasio Tiziana Pepe Paolo Sordino |
| author_facet | Marina Ceruso Celestina Mascolo Pasquale De Luca Iolanda Venuti Giorgio Smaldone Elio Biffali Aniello Anastasio Tiziana Pepe Paolo Sordino |
| author_sort | Marina Ceruso |
| collection | DOAJ |
| description | The commercialization of porgies or seabreams of the family <i>Sparidae</i> has greatly increased in the last decade, and some valuable species have become subject to seafood substitution. DNA regions currently used for fish species identification in fresh and processed products belong to the mitochondrial (mt) genes cytochrome b (<i>Cytb</i>), cytochrome c oxidase I (<i>COI</i>), <i>16S</i> and <i>12S</i>. However, these markers amplify for fragments with lower divergence within and between some species, failing to provide informative barcodes. We adopted comparative mitogenomics, through the analysis of complete mtDNA sequences, as a compatible approach toward studying new barcoding markers. The intent is to develop a specific and rapid assay for the identification of the common pandora <i>Pagellus erythrinus</i>, a sparid species frequently subject to fraudulent replacement. The genetic diversity analysis (Hamming distance, <i>p</i>-genetic distance, gene-by-gene sequence variability) between 16 sparid <i>mt</i>DNA genomes highlighted the discriminating potential of a 291 bp <i>NAD2</i> gene fragment. A pair of species-specific primers were successfully designed and tested by end-point and real-time PCR, achieving amplification only in <i>P. erythrinus</i> among several fish species. The use of the <i>NAD2</i> barcoding marker provides a rapid presence/absence method for the identification of <i>P. erythrinus</i>. |
| first_indexed | 2024-03-10T15:53:05Z |
| format | Article |
| id | doaj.art-9950834a26bc403b9a593a7479c0b4ea |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T15:53:05Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-9950834a26bc403b9a593a7479c0b4ea2023-11-20T15:53:18ZengMDPI AGFoods2304-81582020-10-01910139710.3390/foods9101397A Rapid Method for the Identification of Fresh and Processed <i>Pagellus erythrinus</i> Species against FraudsMarina Ceruso0Celestina Mascolo1Pasquale De Luca2Iolanda Venuti3Giorgio Smaldone4Elio Biffali5Aniello Anastasio6Tiziana Pepe7Paolo Sordino8Department of Veterinary Medicine and Animal Production, University of Naples Federico II, via F. Delpino, n.1, 80137 Naples, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, via F. Delpino, n.1, 80137 Naples, ItalyDepartment of Research Infrastructures for Marine Biological Resources, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, via F. Delpino, n.1, 80137 Naples, ItalyDepartment Agricultural Sciences, University of Naples Federico II, via Università, n.100, Portici, 80055 Naples, ItalyDepartment of Research Infrastructures for Marine Biological Resources, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, via F. Delpino, n.1, 80137 Naples, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, via F. Delpino, n.1, 80137 Naples, ItalyDepartment of Biology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, ItalyThe commercialization of porgies or seabreams of the family <i>Sparidae</i> has greatly increased in the last decade, and some valuable species have become subject to seafood substitution. DNA regions currently used for fish species identification in fresh and processed products belong to the mitochondrial (mt) genes cytochrome b (<i>Cytb</i>), cytochrome c oxidase I (<i>COI</i>), <i>16S</i> and <i>12S</i>. However, these markers amplify for fragments with lower divergence within and between some species, failing to provide informative barcodes. We adopted comparative mitogenomics, through the analysis of complete mtDNA sequences, as a compatible approach toward studying new barcoding markers. The intent is to develop a specific and rapid assay for the identification of the common pandora <i>Pagellus erythrinus</i>, a sparid species frequently subject to fraudulent replacement. The genetic diversity analysis (Hamming distance, <i>p</i>-genetic distance, gene-by-gene sequence variability) between 16 sparid <i>mt</i>DNA genomes highlighted the discriminating potential of a 291 bp <i>NAD2</i> gene fragment. A pair of species-specific primers were successfully designed and tested by end-point and real-time PCR, achieving amplification only in <i>P. erythrinus</i> among several fish species. The use of the <i>NAD2</i> barcoding marker provides a rapid presence/absence method for the identification of <i>P. erythrinus</i>.https://www.mdpi.com/2304-8158/9/10/1397fish species authenticationmtDNAmitogenomicscommon pandora<i>Pagellus erythrinus</i><i>Sparidae</i> |
| spellingShingle | Marina Ceruso Celestina Mascolo Pasquale De Luca Iolanda Venuti Giorgio Smaldone Elio Biffali Aniello Anastasio Tiziana Pepe Paolo Sordino A Rapid Method for the Identification of Fresh and Processed <i>Pagellus erythrinus</i> Species against Frauds Foods fish species authentication mtDNA mitogenomics common pandora <i>Pagellus erythrinus</i> <i>Sparidae</i> |
| title | A Rapid Method for the Identification of Fresh and Processed <i>Pagellus erythrinus</i> Species against Frauds |
| title_full | A Rapid Method for the Identification of Fresh and Processed <i>Pagellus erythrinus</i> Species against Frauds |
| title_fullStr | A Rapid Method for the Identification of Fresh and Processed <i>Pagellus erythrinus</i> Species against Frauds |
| title_full_unstemmed | A Rapid Method for the Identification of Fresh and Processed <i>Pagellus erythrinus</i> Species against Frauds |
| title_short | A Rapid Method for the Identification of Fresh and Processed <i>Pagellus erythrinus</i> Species against Frauds |
| title_sort | rapid method for the identification of fresh and processed i pagellus erythrinus i species against frauds |
| topic | fish species authentication mtDNA mitogenomics common pandora <i>Pagellus erythrinus</i> <i>Sparidae</i> |
| url | https://www.mdpi.com/2304-8158/9/10/1397 |
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