Quantifying Intracellular Viral Pathogen: Specimen Preparation, Visualization and Quantification of Multiple Immunofluorescent Signals in Fixed Human Airway Epithelium Cultured at Air-Liquid Interface

Quantifying Intracellular Viral Pathogen: Specimen Preparation, Visualization and Quantification of Multiple Immunofluorescent Signals in Fixed Human Airway Epithelium Cultured at Air-Liquid Interface

Infection control and aggressive antibiotic therapy play an important role in the management of airway infections in individuals with cystic fibrosis (CF). The responses of airway epithelial cells to pathogens are likely to contribute to the pathobiology of CF lung disease. Primary airway epithelial...

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Bibliographic Details
Main Authors: Sharon L. Wong, Elvis Pandzic, Egi Kardia, Katelin M. Allan, Renee M. Whan, Shafagh A. Waters
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Journal of Personalized Medicine
Subjects:
cystic fibrosis
airway infection
air-liquid interface
wholemount immunofluorescence
tiled imaging
quantification
Online Access:https://www.mdpi.com/2075-4426/12/10/1668

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https://www.mdpi.com/2075-4426/12/10/1668

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