B-cell ELISpot assay to analyze human memory B cell and plasmablast responses specific to SARS-CoV-2 receptor-binding domain

B-cell ELISpot assay to analyze human memory B cell and plasmablast responses specific to SARS-CoV-2 receptor-binding domain

Summary: B-cell ELISpot is an extremely sensitive assay based on the secretion of antibodies by B cells that requires the differentiation of B cells into antibody-secreting cells. Here, we describe the procedure to analyze both plasmablast (PB) and memory B cell (MBC) responses specific to SARS-CoV-...

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Bibliographic Details
Main Authors: Angeline Rouers, Matthew Zirui Tay, Lisa F.P. Ng, Laurent Renia
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Cell Isolation
Cell-based Assays
Health Sciences
Immunology
Antibody
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000886

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http://www.sciencedirect.com/science/article/pii/S2666166723000886

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