Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in Mice
The renal toxicity induced by the intravenously injected iron oxide nanoparticles (IONPs) encapsulated in phospholipid-based polymeric micelles was studied in CD1 mice for 2 weeks. Two doses of 5 and 15 mg of Fe/kg bodyweight of NPs or saline solution (control) were tested, and the levels of antioxi...
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| author | Mihaela Balas Ioana Mihaela Popescu Din Anca Hermenean Ludmila Otilia Cinteza Anca Dinischiotu |
| author_facet | Mihaela Balas Ioana Mihaela Popescu Din Anca Hermenean Ludmila Otilia Cinteza Anca Dinischiotu |
| author_sort | Mihaela Balas |
| collection | DOAJ |
| description | The renal toxicity induced by the intravenously injected iron oxide nanoparticles (IONPs) encapsulated in phospholipid-based polymeric micelles was studied in CD1 mice for 2 weeks. Two doses of 5 and 15 mg of Fe/kg bodyweight of NPs or saline solution (control) were tested, and the levels of antioxidant enzyme activities, oxidative stress parameters, and the expressions of kidney fibrosis biomarkers were analyzed. The enzymatic activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase, and glucose-6-phosphate dehydrogenase in the kidney were significantly decreased compared to the control in the first 3 days followed by a recovery up to 14 days. Concomitantly, a significant increase in lipid peroxidation (malondialdehyde) levels and a decrease in protein thiol groups were recorded. Moreover, increases in the expressions of T cell immunoglobulin and mucin domain 1 (TIM-1) and transforming growth factor-β (TGF-β) were observed in mouse tissue samples in the first week, which were more pronounced for the higher dose. The results suggested the role of oxidative stress as a mechanism for induced toxicity in mice kidneys after the IV administration of IONPs encapsulated in phospholipid-based polymeric micelles but also the capacity of the kidneys’ defense systems to revert efficiently the biochemical modifications that were moderate and for short duration. |
| first_indexed | 2024-03-10T11:21:13Z |
| format | Article |
| id | doaj.art-99af02e91cda43638f37bfa0ebb0f8f8 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T11:21:13Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-99af02e91cda43638f37bfa0ebb0f8f82023-11-21T20:03:28ZengMDPI AGMaterials1996-19442021-05-011410260510.3390/ma14102605Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in MiceMihaela Balas0Ioana Mihaela Popescu Din1Anca Hermenean2Ludmila Otilia Cinteza3Anca Dinischiotu4Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaDepartment of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaDepartment of Experimental and Applied Biology, Institute of Life Sciences, Vasile Goldis Western University of Arad, 86 Rebreanu, 310414 Arad, RomaniaDepartment of Physical Chemistry, Faculty of Chemistry, University of Bucharest, 4-12 Regina Elisabeta Blvd, 030018 Bucharest, RomaniaDepartment of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, RomaniaThe renal toxicity induced by the intravenously injected iron oxide nanoparticles (IONPs) encapsulated in phospholipid-based polymeric micelles was studied in CD1 mice for 2 weeks. Two doses of 5 and 15 mg of Fe/kg bodyweight of NPs or saline solution (control) were tested, and the levels of antioxidant enzyme activities, oxidative stress parameters, and the expressions of kidney fibrosis biomarkers were analyzed. The enzymatic activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase, and glucose-6-phosphate dehydrogenase in the kidney were significantly decreased compared to the control in the first 3 days followed by a recovery up to 14 days. Concomitantly, a significant increase in lipid peroxidation (malondialdehyde) levels and a decrease in protein thiol groups were recorded. Moreover, increases in the expressions of T cell immunoglobulin and mucin domain 1 (TIM-1) and transforming growth factor-β (TGF-β) were observed in mouse tissue samples in the first week, which were more pronounced for the higher dose. The results suggested the role of oxidative stress as a mechanism for induced toxicity in mice kidneys after the IV administration of IONPs encapsulated in phospholipid-based polymeric micelles but also the capacity of the kidneys’ defense systems to revert efficiently the biochemical modifications that were moderate and for short duration.https://www.mdpi.com/1996-1944/14/10/2605iron oxide nanoparticlesphospholipidic micellesoxidative stresskidney fibrosis |
| spellingShingle | Mihaela Balas Ioana Mihaela Popescu Din Anca Hermenean Ludmila Otilia Cinteza Anca Dinischiotu Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in Mice Materials iron oxide nanoparticles phospholipidic micelles oxidative stress kidney fibrosis |
| title | Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in Mice |
| title_full | Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in Mice |
| title_fullStr | Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in Mice |
| title_full_unstemmed | Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in Mice |
| title_short | Exposure to Iron Oxide Nanoparticles Coated with Phospholipid-Based Polymeric Micelles Induces Renal Transitory Biochemical and Histopathological Changes in Mice |
| title_sort | exposure to iron oxide nanoparticles coated with phospholipid based polymeric micelles induces renal transitory biochemical and histopathological changes in mice |
| topic | iron oxide nanoparticles phospholipidic micelles oxidative stress kidney fibrosis |
| url | https://www.mdpi.com/1996-1944/14/10/2605 |
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