Annealing and purification of fluorescently labeled DNA substrates for in vitro assays

Annealing and purification of fluorescently labeled DNA substrates for in vitro assays

Summary: We present a protocol to generate high-quality fluorescently labeled DNA substrates that can be used for biochemical assays, including DNA-binding and nuclease activity assays. We describe polyacrylamide-gel-electrophoresis-based purification of DNA oligonucleotides, followed by annealing t...

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Bibliographic Details
Main Authors: Ying Wah Elizabeth Tse, Hwa Young Yun, Haley Doris Myskiw Wyatt
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Molecular Biology
Molecular/Chemical Probes
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723000862

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723000862

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