Acute and continuous exposure of airborne fine particulate matter (PM2.5): diverse outer blood–retinal barrier damages and disease susceptibilities
Abstract Background The association between air pollution and retinal diseases such as age-related macular degeneration (AMD) has been demonstrated, but the pathogenic correlation is unknown. Damage to the outer blood–retinal barrier (oBRB), which consists of the retinal pigment epithelium (RPE) and...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Particle and Fibre Toxicology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12989-023-00558-2 |
| _version_ | 1797377210975256576 |
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| author | Yuzhou Gu Feiyin Sheng Mengqin Gao Li Zhang Shengjie Hao Shuying Chen Rongrong Chen Yili Xu Di Wu Yu Han Lu Chen Ye Liu Bing Lu Wei Zhao Xiaoming Lou Zhijian Chen Peng Li Xiaofeng Wang Ke Yao Qiuli Fu |
| author_facet | Yuzhou Gu Feiyin Sheng Mengqin Gao Li Zhang Shengjie Hao Shuying Chen Rongrong Chen Yili Xu Di Wu Yu Han Lu Chen Ye Liu Bing Lu Wei Zhao Xiaoming Lou Zhijian Chen Peng Li Xiaofeng Wang Ke Yao Qiuli Fu |
| author_sort | Yuzhou Gu |
| collection | DOAJ |
| description | Abstract Background The association between air pollution and retinal diseases such as age-related macular degeneration (AMD) has been demonstrated, but the pathogenic correlation is unknown. Damage to the outer blood–retinal barrier (oBRB), which consists of the retinal pigment epithelium (RPE) and choriocapillaris, is crucial in the development of fundus diseases. Objectives To describe the effects of airborne fine particulate matter (PM2.5) on the oBRB and disease susceptibilities. Methods A PM2.5-exposed mice model was established through the administration of eye drops containing PM2.5. Optical coherence tomography angiography, transmission electron microscope, RPE immunofluorescence staining and Western blotting were applied to study the oBRB changes. A co-culture model of ARPE-19 cells with stretching vascular endothelial cells was established to identify the role of choroidal vasodilatation in PM2.5-associated RPE damage. Results Acute exposure to PM2.5 resulted in choroidal vasodilatation, RPE tight junctions impairment, and ultimately an increased risk of retinal edema in mice. These manifestations are very similar to the pachychoroid disease represented by central serous chorioretinopathy (CSC). After continuous PM2.5 exposure, the damage to the RPE was gradually repaired, but AMD-related early retinal degenerative changes appeared under continuous choroidal inflammation. Conclusion This study reveals oBRB pathological changes under different exposure durations, providing a valuable reference for the prevention of PM2.5-related fundus diseases and public health policy formulation. Graphical abstract |
| first_indexed | 2024-03-08T19:50:28Z |
| format | Article |
| id | doaj.art-99b0839401884236a0ffb8849877896f |
| institution | Directory Open Access Journal |
| issn | 1743-8977 |
| language | English |
| last_indexed | 2024-03-08T19:50:28Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Particle and Fibre Toxicology |
| spelling | doaj.art-99b0839401884236a0ffb8849877896f2023-12-24T12:08:11ZengBMCParticle and Fibre Toxicology1743-89772023-12-0120111810.1186/s12989-023-00558-2Acute and continuous exposure of airborne fine particulate matter (PM2.5): diverse outer blood–retinal barrier damages and disease susceptibilitiesYuzhou Gu0Feiyin Sheng1Mengqin Gao2Li Zhang3Shengjie Hao4Shuying Chen5Rongrong Chen6Yili Xu7Di Wu8Yu Han9Lu Chen10Ye Liu11Bing Lu12Wei Zhao13Xiaoming Lou14Zhijian Chen15Peng Li16Xiaofeng Wang17Ke Yao18Qiuli Fu19Eye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyState Key Lab of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang UniversityEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyDepartment of Environmental and Occupational Health, Zhejiang Provincial Center for Disease Control and PreventionDepartment of Environmental and Occupational Health, Zhejiang Provincial Center for Disease Control and PreventionState Key Lab of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang UniversityDepartment of Environmental and Occupational Health, Zhejiang Provincial Center for Disease Control and PreventionEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyEye Center of the 2nd Affiliated Hospital, School of Medicine, Medical College of Zhejiang University, Zhejiang University, Zhejiang Provincial Key Lab of OphthalmologyAbstract Background The association between air pollution and retinal diseases such as age-related macular degeneration (AMD) has been demonstrated, but the pathogenic correlation is unknown. Damage to the outer blood–retinal barrier (oBRB), which consists of the retinal pigment epithelium (RPE) and choriocapillaris, is crucial in the development of fundus diseases. Objectives To describe the effects of airborne fine particulate matter (PM2.5) on the oBRB and disease susceptibilities. Methods A PM2.5-exposed mice model was established through the administration of eye drops containing PM2.5. Optical coherence tomography angiography, transmission electron microscope, RPE immunofluorescence staining and Western blotting were applied to study the oBRB changes. A co-culture model of ARPE-19 cells with stretching vascular endothelial cells was established to identify the role of choroidal vasodilatation in PM2.5-associated RPE damage. Results Acute exposure to PM2.5 resulted in choroidal vasodilatation, RPE tight junctions impairment, and ultimately an increased risk of retinal edema in mice. These manifestations are very similar to the pachychoroid disease represented by central serous chorioretinopathy (CSC). After continuous PM2.5 exposure, the damage to the RPE was gradually repaired, but AMD-related early retinal degenerative changes appeared under continuous choroidal inflammation. Conclusion This study reveals oBRB pathological changes under different exposure durations, providing a valuable reference for the prevention of PM2.5-related fundus diseases and public health policy formulation. Graphical abstracthttps://doi.org/10.1186/s12989-023-00558-2PM2.5Retinal pigment epitheliumChoriocapillarisInflammationOuter blood–retinal barrier |
| spellingShingle | Yuzhou Gu Feiyin Sheng Mengqin Gao Li Zhang Shengjie Hao Shuying Chen Rongrong Chen Yili Xu Di Wu Yu Han Lu Chen Ye Liu Bing Lu Wei Zhao Xiaoming Lou Zhijian Chen Peng Li Xiaofeng Wang Ke Yao Qiuli Fu Acute and continuous exposure of airborne fine particulate matter (PM2.5): diverse outer blood–retinal barrier damages and disease susceptibilities Particle and Fibre Toxicology PM2.5 Retinal pigment epithelium Choriocapillaris Inflammation Outer blood–retinal barrier |
| title | Acute and continuous exposure of airborne fine particulate matter (PM2.5): diverse outer blood–retinal barrier damages and disease susceptibilities |
| title_full | Acute and continuous exposure of airborne fine particulate matter (PM2.5): diverse outer blood–retinal barrier damages and disease susceptibilities |
| title_fullStr | Acute and continuous exposure of airborne fine particulate matter (PM2.5): diverse outer blood–retinal barrier damages and disease susceptibilities |
| title_full_unstemmed | Acute and continuous exposure of airborne fine particulate matter (PM2.5): diverse outer blood–retinal barrier damages and disease susceptibilities |
| title_short | Acute and continuous exposure of airborne fine particulate matter (PM2.5): diverse outer blood–retinal barrier damages and disease susceptibilities |
| title_sort | acute and continuous exposure of airborne fine particulate matter pm2 5 diverse outer blood retinal barrier damages and disease susceptibilities |
| topic | PM2.5 Retinal pigment epithelium Choriocapillaris Inflammation Outer blood–retinal barrier |
| url | https://doi.org/10.1186/s12989-023-00558-2 |
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