Effects of Light Orientation and Mechanical Damage to Leaves on Isoflavone Accumulation in Soybean Seeds
Soybean is largely cultivated worldwide providing high amounts of proteins and oil for food and feed, and isoflavones for nutraceutical uses. The increasing interest in agroforestry practices for improving carbon sequestration and mitigating climate changes suggests the need to assess soybean respon...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/11/3/589 |
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| author | Giuseppe Barion Mohamed Hewidy Anna Panozzo Andrea Aloia Teofilo Vamerali |
| author_facet | Giuseppe Barion Mohamed Hewidy Anna Panozzo Andrea Aloia Teofilo Vamerali |
| author_sort | Giuseppe Barion |
| collection | DOAJ |
| description | Soybean is largely cultivated worldwide providing high amounts of proteins and oil for food and feed, and isoflavones for nutraceutical uses. The increasing interest in agroforestry practices for improving carbon sequestration and mitigating climate changes suggests the need to assess soybean response to variations of light availability and direction. A two-year pot trial was carried out at Legnaro (NE Italy) in order to mimic the response of the soybean var. Sponsor to contrasting light orientation (east or west) by artificial shading, associated or not with mechanical leaf damage, in terms of protein accumulation, total cotyledon isoflavone concentration (TCIC) and isoflavone profile. Here, we demonstrate that a different intensity of the isoflavone metabolism exists in response to lighting conditions, with higher TCIC and slightly increased seed crude proteins in plants lighted from the east (morning time) and after mechanical leaf damage. The isoflavone profile was not changed, but low temperatures and high rainfall during seed filling (1st year) were associated with increased accumulation of medium-high molecular weight (MW) forms (i.e., glycosyls and malonyls), while high temperatures and low rainfall (2nd year) with increased accumulation of medium-low MW forms (i.e., glycosyls and aglycones). It is concluded that within agroforestry systems, there is possibly a large scope for maximizing isoflavone accumulation by selecting the harvesting area in the neighboring of the east side of the tree alleys, with further improvements if a mild shoot stripping is applied before flowering. |
| first_indexed | 2024-03-10T13:04:01Z |
| format | Article |
| id | doaj.art-99b270f30d9e4194bdfa9a9250e32a48 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T13:04:01Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-99b270f30d9e4194bdfa9a9250e32a482023-11-21T11:16:33ZengMDPI AGAgronomy2073-43952021-03-0111358910.3390/agronomy11030589Effects of Light Orientation and Mechanical Damage to Leaves on Isoflavone Accumulation in Soybean SeedsGiuseppe Barion0Mohamed Hewidy1Anna Panozzo2Andrea Aloia3Teofilo Vamerali4Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padua, Viale dell’ Università 16, Legnaro, 35020 Padova, ItalyDepartment of Horticulture, Faculty of Agriculture, University of Ain Shams, Hadayek Shoubra 68, Cairo 11241, EgyptDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padua, Viale dell’ Università 16, Legnaro, 35020 Padova, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padua, Viale dell’ Università 16, Legnaro, 35020 Padova, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment, University of Padua, Viale dell’ Università 16, Legnaro, 35020 Padova, ItalySoybean is largely cultivated worldwide providing high amounts of proteins and oil for food and feed, and isoflavones for nutraceutical uses. The increasing interest in agroforestry practices for improving carbon sequestration and mitigating climate changes suggests the need to assess soybean response to variations of light availability and direction. A two-year pot trial was carried out at Legnaro (NE Italy) in order to mimic the response of the soybean var. Sponsor to contrasting light orientation (east or west) by artificial shading, associated or not with mechanical leaf damage, in terms of protein accumulation, total cotyledon isoflavone concentration (TCIC) and isoflavone profile. Here, we demonstrate that a different intensity of the isoflavone metabolism exists in response to lighting conditions, with higher TCIC and slightly increased seed crude proteins in plants lighted from the east (morning time) and after mechanical leaf damage. The isoflavone profile was not changed, but low temperatures and high rainfall during seed filling (1st year) were associated with increased accumulation of medium-high molecular weight (MW) forms (i.e., glycosyls and malonyls), while high temperatures and low rainfall (2nd year) with increased accumulation of medium-low MW forms (i.e., glycosyls and aglycones). It is concluded that within agroforestry systems, there is possibly a large scope for maximizing isoflavone accumulation by selecting the harvesting area in the neighboring of the east side of the tree alleys, with further improvements if a mild shoot stripping is applied before flowering.https://www.mdpi.com/2073-4395/11/3/589agroforestrylight orientationsoybeanisoflavonesmechanical stress |
| spellingShingle | Giuseppe Barion Mohamed Hewidy Anna Panozzo Andrea Aloia Teofilo Vamerali Effects of Light Orientation and Mechanical Damage to Leaves on Isoflavone Accumulation in Soybean Seeds Agronomy agroforestry light orientation soybean isoflavones mechanical stress |
| title | Effects of Light Orientation and Mechanical Damage to Leaves on Isoflavone Accumulation in Soybean Seeds |
| title_full | Effects of Light Orientation and Mechanical Damage to Leaves on Isoflavone Accumulation in Soybean Seeds |
| title_fullStr | Effects of Light Orientation and Mechanical Damage to Leaves on Isoflavone Accumulation in Soybean Seeds |
| title_full_unstemmed | Effects of Light Orientation and Mechanical Damage to Leaves on Isoflavone Accumulation in Soybean Seeds |
| title_short | Effects of Light Orientation and Mechanical Damage to Leaves on Isoflavone Accumulation in Soybean Seeds |
| title_sort | effects of light orientation and mechanical damage to leaves on isoflavone accumulation in soybean seeds |
| topic | agroforestry light orientation soybean isoflavones mechanical stress |
| url | https://www.mdpi.com/2073-4395/11/3/589 |
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