Effect of Processing Treatment and Modified Atmosphere Packing on Carrot’s Microbial Community Structure by Illumina MiSeq Sequencing
The aim of this study was to analyze the microbiome of carrot (<i>Daucus carota</i> subsp. <i>sativus</i>) subjected to minimal pre-treatment (rinsing in organic acid solution) and packaging in a high-oxygen modified atmosphere, and then stored for 17 days under refrigeration...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/9/2830 |
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| author | Katarzyna Ratajczak Justyna Staninska-Pięta Jakub Czarny Paweł Cyplik Łukasz Wolko Agnieszka Piotrowska-Cyplik |
| author_facet | Katarzyna Ratajczak Justyna Staninska-Pięta Jakub Czarny Paweł Cyplik Łukasz Wolko Agnieszka Piotrowska-Cyplik |
| author_sort | Katarzyna Ratajczak |
| collection | DOAJ |
| description | The aim of this study was to analyze the microbiome of carrot (<i>Daucus carota</i> subsp. <i>sativus</i>) subjected to minimal pre-treatment (rinsing in organic acid solution) and packaging in a high-oxygen modified atmosphere, and then stored for 17 days under refrigeration conditions (4 °C). The highest levels of bacteria in the carrot microbiome were characterized, at almost 78%, by bacteria belonging to the Enterobacteriaceae and Pseudomonadaceae families. Rinsing in a solution of ascorbic and citric acids resulted in the improvement of microbiological quality in the first day of storage. However, the use of a high-oxygen modified atmosphere extended the shelf life of the minimally processed product. Compared to carrots stored in air, those stored in high oxygen concentration were characterized by a greater ratio of bacteria belonging to the <i>Serratia</i> and <i>Enterobacter</i> genera, and a lower ratio belonging to the <i>Pseudomonas</i> and <i>Pantoea</i> genera. Moreover, the β-biodiversity analysis confirmed that the oxygen concentration was the main factor influencing the differentiation of the metabiomes of the stored carrots. The bacterial strains isolated from carrots identified by molecular methods were mostly pathogenic or potentially pathogenic microorganisms. Neither the minimal pre-treatment nor packaging in high-oxygen atmosphere was able to eliminate the threat of pathogenic bacteria emerging in the product. |
| first_indexed | 2024-03-10T03:53:52Z |
| format | Article |
| id | doaj.art-9a091dd6c01c48dd952644e6715cc552 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:53:52Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9a091dd6c01c48dd952644e6715cc5522023-11-23T08:50:17ZengMDPI AGMolecules1420-30492022-04-01279283010.3390/molecules27092830Effect of Processing Treatment and Modified Atmosphere Packing on Carrot’s Microbial Community Structure by Illumina MiSeq SequencingKatarzyna Ratajczak0Justyna Staninska-Pięta1Jakub Czarny2Paweł Cyplik3Łukasz Wolko4Agnieszka Piotrowska-Cyplik5Department of Food Technology of Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandDepartment of Food Technology of Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandInstitute of Forensic Genetics, Al. Mickiewicza 3/4, 85-071 Bydgoszcz, PolandDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, PolandDepartment of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznań, PolandDepartment of Food Technology of Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandThe aim of this study was to analyze the microbiome of carrot (<i>Daucus carota</i> subsp. <i>sativus</i>) subjected to minimal pre-treatment (rinsing in organic acid solution) and packaging in a high-oxygen modified atmosphere, and then stored for 17 days under refrigeration conditions (4 °C). The highest levels of bacteria in the carrot microbiome were characterized, at almost 78%, by bacteria belonging to the Enterobacteriaceae and Pseudomonadaceae families. Rinsing in a solution of ascorbic and citric acids resulted in the improvement of microbiological quality in the first day of storage. However, the use of a high-oxygen modified atmosphere extended the shelf life of the minimally processed product. Compared to carrots stored in air, those stored in high oxygen concentration were characterized by a greater ratio of bacteria belonging to the <i>Serratia</i> and <i>Enterobacter</i> genera, and a lower ratio belonging to the <i>Pseudomonas</i> and <i>Pantoea</i> genera. Moreover, the β-biodiversity analysis confirmed that the oxygen concentration was the main factor influencing the differentiation of the metabiomes of the stored carrots. The bacterial strains isolated from carrots identified by molecular methods were mostly pathogenic or potentially pathogenic microorganisms. Neither the minimal pre-treatment nor packaging in high-oxygen atmosphere was able to eliminate the threat of pathogenic bacteria emerging in the product.https://www.mdpi.com/1420-3049/27/9/2830microbiome of carrotmodified atmosphere packagingspecific spoilage organisms |
| spellingShingle | Katarzyna Ratajczak Justyna Staninska-Pięta Jakub Czarny Paweł Cyplik Łukasz Wolko Agnieszka Piotrowska-Cyplik Effect of Processing Treatment and Modified Atmosphere Packing on Carrot’s Microbial Community Structure by Illumina MiSeq Sequencing Molecules microbiome of carrot modified atmosphere packaging specific spoilage organisms |
| title | Effect of Processing Treatment and Modified Atmosphere Packing on Carrot’s Microbial Community Structure by Illumina MiSeq Sequencing |
| title_full | Effect of Processing Treatment and Modified Atmosphere Packing on Carrot’s Microbial Community Structure by Illumina MiSeq Sequencing |
| title_fullStr | Effect of Processing Treatment and Modified Atmosphere Packing on Carrot’s Microbial Community Structure by Illumina MiSeq Sequencing |
| title_full_unstemmed | Effect of Processing Treatment and Modified Atmosphere Packing on Carrot’s Microbial Community Structure by Illumina MiSeq Sequencing |
| title_short | Effect of Processing Treatment and Modified Atmosphere Packing on Carrot’s Microbial Community Structure by Illumina MiSeq Sequencing |
| title_sort | effect of processing treatment and modified atmosphere packing on carrot s microbial community structure by illumina miseq sequencing |
| topic | microbiome of carrot modified atmosphere packaging specific spoilage organisms |
| url | https://www.mdpi.com/1420-3049/27/9/2830 |
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