Evaluation of 16S rRNA broad range PCR assay for microbial detection in serum specimens in sepsis patients

Evaluation of 16S rRNA broad range PCR assay for microbial detection in serum specimens in sepsis patients

Background: Early and accurate laboratory diagnosis and appropriate management of infection improves the survival rate in sepsis. In this study we evaluated broad range 16S rRNA and 16 S–23 S intergenic spacer region (ISR) PCR assays followed by nucleotide sequencing directly from patients’ serum an...

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Bibliographic Details
Main Authors: Deepanshi Mishra, Gita Satpathy, Naveet Wig, Farhan Fazal, Nishat Hussain Ahmed, Subrat Kumar Panda
Format: Article
Language:English
Published: Elsevier 2020-07-01
Series:Journal of Infection and Public Health
Subjects:
Broad range PCR assay
Automated culture
Nucleotide sequencing
NCBI BLAST analysis
Sepsis
Blood stream infection
Online Access:http://www.sciencedirect.com/science/article/pii/S1876034120300071

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http://www.sciencedirect.com/science/article/pii/S1876034120300071

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