Precise isolation of high-quality RNA from leaves and storage roots of cassava (Manihot esculenta Crantz) for gene expression studies

Precise isolation of high-quality RNA from leaves and storage roots of cassava (Manihot esculenta Crantz) for gene expression studies

Abstract Background The isolation of Ribonucleic Acid (RNA) from leaves and storage roots of cassava (Manihot esculenta Crantz) is a challenging one, due to the presence of large amounts of polyphenolic compounds, polysaccharides, and tuber proteins. RNA with high quality and intact integrity is vit...

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Bibliographic Details
Main Authors: Jane Ijeoma Reuben-Kalu, Kokiladevi Eswaran, Raveendran Muthurajan, Uma Doraiswamy, Balasubramani Venkatasamy, Kavitha Periannagounder Shanmugam
Format: Article
Language:English
Published: SpringerOpen 2023-06-01
Series:Bulletin of the National Research Centre
Subjects:
Cassava (Manihot esculenta Crantz)
Leaf and storage root
RNA isolation
Polyphenols
Polysaccharides
RT-qPCR
Online Access:https://doi.org/10.1186/s42269-023-01058-1

Internet

https://doi.org/10.1186/s42269-023-01058-1

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