Use of quantitative mass spectrometry-based proteomics and ELISA to compare the alpha 2 macroglobulin concentration in equine blood-based products processed by three different orthobiologic devices
IntroductionAlpha 2 macroglobulin (A2M), a multi-functional protein in the plasma protease inhibitor class, regulates proinflammatory cytokines and the clearance of chondrodestructive enzymes in cases of joint injury and osteoarthritis (OA). The purpose of this study was to compare A2M concentration...
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1335972/full |
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| author | Kyla F. Ortved Larry Alward Bobby Cowles Renata Linardi Dhvani Barot Alex Usimaki Joseph R. Fedie Deb Amodie Laurie R. Goodrich |
| author_facet | Kyla F. Ortved Larry Alward Bobby Cowles Renata Linardi Dhvani Barot Alex Usimaki Joseph R. Fedie Deb Amodie Laurie R. Goodrich |
| author_sort | Kyla F. Ortved |
| collection | DOAJ |
| description | IntroductionAlpha 2 macroglobulin (A2M), a multi-functional protein in the plasma protease inhibitor class, regulates proinflammatory cytokines and the clearance of chondrodestructive enzymes in cases of joint injury and osteoarthritis (OA). The purpose of this study was to compare A2M concentrations in equine plasma samples processed by three commercial devices developed for stall-side regenerative joint therapy.MethodsPlasma samples were obtained from healthy adult horses (N = 13). Mass spectrometry analysis was used to determine the concentration of protein analytes in each sample. Selected reaction monitoring measured a specific A2M peptide as a surrogate of the whole A2M protein. A2M concentrations produced by each test device were compared for two sample types: a pre-concentrate or platelet-poor (PP) component and a final component for use in the horse.ResultsThere was no significant difference (p > 0.05) in the geometric mean (GM) concentration of A2M in the final concentration samples produced by the Alpha2EQ® device (N horses = 13) and the single-centrifugation PP samples produced by the Pro-Stride® APS (autologous protein solution) device (N = 13) and the Restigen® PRP (platelet-rich plasma) device (N = 11). When A2M content in final concentration samples produced by each device was compared, the Pro-Stride APS and Restigen PRP samples had significantly greater GM A2M content (p < 0.0001) compared to the Alpha2EQ samples, and the Pro-Stride APS final concentration samples had significantly greater GM A2M concentration (p < 0.0001) versus that for the Restigen PRP final samples.DiscussionThis comparison demonstrated that the volume and A2M concentration of an Alpha2EQ final concentrate are no different than the volume and concentration of A2M in the PP from Pro-Stride or Restigen devices. |
| first_indexed | 2024-03-08T04:06:44Z |
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| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-08T04:06:44Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-9a3a933dea9042be92ebf3e7fe17f8a62024-02-09T04:53:22ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-02-011110.3389/fvets.2024.13359721335972Use of quantitative mass spectrometry-based proteomics and ELISA to compare the alpha 2 macroglobulin concentration in equine blood-based products processed by three different orthobiologic devicesKyla F. Ortved0Larry Alward1Bobby Cowles2Renata Linardi3Dhvani Barot4Alex Usimaki5Joseph R. Fedie6Deb Amodie7Laurie R. Goodrich8New Bolton Center, University of Pennsylvania, Kennett, PA, United StatesVeterinary Medicine Research and Development, Zoetis, Kalamazoo, MI, United StatesEquine Technical Services, Zoetis, Parsippany, NJ, United StatesNew Bolton Center, University of Pennsylvania, Kennett, PA, United StatesNew Bolton Center, University of Pennsylvania, Kennett, PA, United StatesNew Bolton Center, University of Pennsylvania, Kennett, PA, United StatesVeterinary Medicine Research and Development, Zoetis, Kalamazoo, MI, United StatesOutcomes Research, Zoetis, Parsippany, NJ, United StatesOrthopaedic Research Center, Translational Medicine Institute, College of Veterinary Medicine and Biomedical Science, Colorado State University, Ft Collins, CO, United StatesIntroductionAlpha 2 macroglobulin (A2M), a multi-functional protein in the plasma protease inhibitor class, regulates proinflammatory cytokines and the clearance of chondrodestructive enzymes in cases of joint injury and osteoarthritis (OA). The purpose of this study was to compare A2M concentrations in equine plasma samples processed by three commercial devices developed for stall-side regenerative joint therapy.MethodsPlasma samples were obtained from healthy adult horses (N = 13). Mass spectrometry analysis was used to determine the concentration of protein analytes in each sample. Selected reaction monitoring measured a specific A2M peptide as a surrogate of the whole A2M protein. A2M concentrations produced by each test device were compared for two sample types: a pre-concentrate or platelet-poor (PP) component and a final component for use in the horse.ResultsThere was no significant difference (p > 0.05) in the geometric mean (GM) concentration of A2M in the final concentration samples produced by the Alpha2EQ® device (N horses = 13) and the single-centrifugation PP samples produced by the Pro-Stride® APS (autologous protein solution) device (N = 13) and the Restigen® PRP (platelet-rich plasma) device (N = 11). When A2M content in final concentration samples produced by each device was compared, the Pro-Stride APS and Restigen PRP samples had significantly greater GM A2M content (p < 0.0001) compared to the Alpha2EQ samples, and the Pro-Stride APS final concentration samples had significantly greater GM A2M concentration (p < 0.0001) versus that for the Restigen PRP final samples.DiscussionThis comparison demonstrated that the volume and A2M concentration of an Alpha2EQ final concentrate are no different than the volume and concentration of A2M in the PP from Pro-Stride or Restigen devices.https://www.frontiersin.org/articles/10.3389/fvets.2024.1335972/fullalpha 2 macroglobulinequineprotease inhibitorproteomicregenerative joint disease therapyselected reaction monitoring |
| spellingShingle | Kyla F. Ortved Larry Alward Bobby Cowles Renata Linardi Dhvani Barot Alex Usimaki Joseph R. Fedie Deb Amodie Laurie R. Goodrich Use of quantitative mass spectrometry-based proteomics and ELISA to compare the alpha 2 macroglobulin concentration in equine blood-based products processed by three different orthobiologic devices Frontiers in Veterinary Science alpha 2 macroglobulin equine protease inhibitor proteomic regenerative joint disease therapy selected reaction monitoring |
| title | Use of quantitative mass spectrometry-based proteomics and ELISA to compare the alpha 2 macroglobulin concentration in equine blood-based products processed by three different orthobiologic devices |
| title_full | Use of quantitative mass spectrometry-based proteomics and ELISA to compare the alpha 2 macroglobulin concentration in equine blood-based products processed by three different orthobiologic devices |
| title_fullStr | Use of quantitative mass spectrometry-based proteomics and ELISA to compare the alpha 2 macroglobulin concentration in equine blood-based products processed by three different orthobiologic devices |
| title_full_unstemmed | Use of quantitative mass spectrometry-based proteomics and ELISA to compare the alpha 2 macroglobulin concentration in equine blood-based products processed by three different orthobiologic devices |
| title_short | Use of quantitative mass spectrometry-based proteomics and ELISA to compare the alpha 2 macroglobulin concentration in equine blood-based products processed by three different orthobiologic devices |
| title_sort | use of quantitative mass spectrometry based proteomics and elisa to compare the alpha 2 macroglobulin concentration in equine blood based products processed by three different orthobiologic devices |
| topic | alpha 2 macroglobulin equine protease inhibitor proteomic regenerative joint disease therapy selected reaction monitoring |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1335972/full |
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