Use of tannase‐producing bacteria isolated from the rumen to improve the nutritional value of pomegranate peel for fattening lambs

Abstract Background The use of plants and by‐products, which are containing a high amount of secondary and anti‐nutritional compounds such as tannins, in animal feed is limited. The methods that can reduce these compounds make facilitate their use in animal feed. Objectives The aim of this study was...

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Main Authors: Morteza Chaji, Zahra Jahanara
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Veterinary Medicine and Science
Subjects:
Online Access:https://doi.org/10.1002/vms3.1347
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author Morteza Chaji
Zahra Jahanara
author_facet Morteza Chaji
Zahra Jahanara
author_sort Morteza Chaji
collection DOAJ
description Abstract Background The use of plants and by‐products, which are containing a high amount of secondary and anti‐nutritional compounds such as tannins, in animal feed is limited. The methods that can reduce these compounds make facilitate their use in animal feed. Objectives The aim of this study was to reduce the adverse effects of pomegranate peel (PP) tannin for fattening lambs using the tannase‐producing bacteria. Methods Twenty‐one Arabi male lambs (averagely 35 ± 3.8 kg weight and 8 ± 1.0 months age) were used in a completely randomized design with three treatments and seven replications in the present experiment. The experimental treatments included 1 – control diet (CNT, no PP), 2 – diet containing untreated PP (raw PP, UTPP) and 3 – diet containing PP treated with tannase‐producing bacteria (bacteria treating PP, BTPP). Results Using UTPP decreased nutrient intake compared to the control and treatment with tannase‐producing bacteria again significantly increased nutrient intake compared to the UTPP (p < 0.05). The digestibilities of organic matter, neutral detergent fibre and acid detergent fibre in the control treatment were significantly higher than UTPP and BTPP and in the BTPP were significantly higher than the UTPP (p < 0.05). The use of UTPP in the diet significantly decreased the pH, ammonia nitrogen concentration and the total protozoa population of the rumen compared to the control (p < 0.05), and treatment with bacteria increased them again. The lowest total protozoa population was observed in UTPP treatments (p < 0.05). The highest concentration of blood glucose was observed in UTPP; however, the highest concentrations of blood urea nitrogen, cholesterol, triglyceride, high‐density lipoprotein (non‐significant) and low‐density lipoprotein were in the control treatment. The effect of experimental treatments on the dry matter consumption of the whole period was significant; however, there was no significant effect on average daily gain, feed conversion ratio, feed efficiency and longissimus muscle colorimetric systems. Conclusions Therefore, considering the positive effects of treatment PP with tannin‐degrading bacteria relative to raw PP, using these bacteria is a proper way to reduce tannin, thus improving the nutritional value of PP for ruminants.
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spelling doaj.art-9a5e1860ae704b29bd35598271be51442024-01-16T18:58:33ZengWileyVeterinary Medicine and Science2053-10952024-01-01101n/an/a10.1002/vms3.1347Use of tannase‐producing bacteria isolated from the rumen to improve the nutritional value of pomegranate peel for fattening lambsMorteza Chaji0Zahra Jahanara1Department of Animal Science Faculty of Animal Science and Food Technology Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani Ahvaz Khuzestan IranDepartment of Animal Science Faculty of Animal Science and Food Technology Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani Ahvaz Khuzestan IranAbstract Background The use of plants and by‐products, which are containing a high amount of secondary and anti‐nutritional compounds such as tannins, in animal feed is limited. The methods that can reduce these compounds make facilitate their use in animal feed. Objectives The aim of this study was to reduce the adverse effects of pomegranate peel (PP) tannin for fattening lambs using the tannase‐producing bacteria. Methods Twenty‐one Arabi male lambs (averagely 35 ± 3.8 kg weight and 8 ± 1.0 months age) were used in a completely randomized design with three treatments and seven replications in the present experiment. The experimental treatments included 1 – control diet (CNT, no PP), 2 – diet containing untreated PP (raw PP, UTPP) and 3 – diet containing PP treated with tannase‐producing bacteria (bacteria treating PP, BTPP). Results Using UTPP decreased nutrient intake compared to the control and treatment with tannase‐producing bacteria again significantly increased nutrient intake compared to the UTPP (p < 0.05). The digestibilities of organic matter, neutral detergent fibre and acid detergent fibre in the control treatment were significantly higher than UTPP and BTPP and in the BTPP were significantly higher than the UTPP (p < 0.05). The use of UTPP in the diet significantly decreased the pH, ammonia nitrogen concentration and the total protozoa population of the rumen compared to the control (p < 0.05), and treatment with bacteria increased them again. The lowest total protozoa population was observed in UTPP treatments (p < 0.05). The highest concentration of blood glucose was observed in UTPP; however, the highest concentrations of blood urea nitrogen, cholesterol, triglyceride, high‐density lipoprotein (non‐significant) and low‐density lipoprotein were in the control treatment. The effect of experimental treatments on the dry matter consumption of the whole period was significant; however, there was no significant effect on average daily gain, feed conversion ratio, feed efficiency and longissimus muscle colorimetric systems. Conclusions Therefore, considering the positive effects of treatment PP with tannin‐degrading bacteria relative to raw PP, using these bacteria is a proper way to reduce tannin, thus improving the nutritional value of PP for ruminants.https://doi.org/10.1002/vms3.1347arabi lambsblood metabolitesrumen parametersTannin
spellingShingle Morteza Chaji
Zahra Jahanara
Use of tannase‐producing bacteria isolated from the rumen to improve the nutritional value of pomegranate peel for fattening lambs
Veterinary Medicine and Science
arabi lambs
blood metabolites
rumen parameters
Tannin
title Use of tannase‐producing bacteria isolated from the rumen to improve the nutritional value of pomegranate peel for fattening lambs
title_full Use of tannase‐producing bacteria isolated from the rumen to improve the nutritional value of pomegranate peel for fattening lambs
title_fullStr Use of tannase‐producing bacteria isolated from the rumen to improve the nutritional value of pomegranate peel for fattening lambs
title_full_unstemmed Use of tannase‐producing bacteria isolated from the rumen to improve the nutritional value of pomegranate peel for fattening lambs
title_short Use of tannase‐producing bacteria isolated from the rumen to improve the nutritional value of pomegranate peel for fattening lambs
title_sort use of tannase producing bacteria isolated from the rumen to improve the nutritional value of pomegranate peel for fattening lambs
topic arabi lambs
blood metabolites
rumen parameters
Tannin
url https://doi.org/10.1002/vms3.1347
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