Generation of αMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting

Generation of αMHC-EGFP knock-in in human pluripotent stem cell line, SNUe003-A-3 using CRISPR/Cas9-based gene targeting

The cardiac muscle-specific protein, α-myosin heavy chain (αMHC), is a major component of cardiac muscle filaments involved in cardiac muscle contraction. Here, we established an αMHC-enhanced fluorescent protein (EGFP) knock-in human pluripotent stem cell (hPSC) line by linking the EGFP gene to the...

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Bibliographic Details
Main Authors: Ha Myoung Lee, A-Hyeon Kim, Sunsook Hwang, Juwon Jung, Hyewon Seol, Jin Jea Sung, Seung Min Jeong, Young Min Choi, Jong Kwan Jun, Han-Soo Kim, Jiho Jang
Format: Article
Language:English
Published: Elsevier 2022-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506122001283

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http://www.sciencedirect.com/science/article/pii/S1873506122001283

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