Generation of NANOS3-mCherry reporter human embryonic stem cell line SYSUe-009-A using CRISPR/Cas9

NANOS3 is a zinc-finger containing RNA-binding protein that has been demonstrated to be highly expressed in human primordial germ cell(hPGC), thus NANOS3 can serve as a marker for hPGC development. Due to the ethical and technical restrictions, it is difficult to get primary human germline cells. Hu...

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Bibliographic Details
Main Authors: Ruirui Guo, Simin Yuan, Bing Li, Jichang Wang, Chuanbo Sun
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506123000089