Automated Cell Treatment for Competence and Transformation of Escherichia coli in a High-Throughput Quasi-Turbidostat Using Microtiter Plates

Automated Cell Treatment for Competence and Transformation of Escherichia coli in a High-Throughput Quasi-Turbidostat Using Microtiter Plates

Metabolic engineering and genome editing strategies often lead to large strain libraries of a bacterial host. Nevertheless, the generation of competent cells is the basis for transformation and subsequent screening of these strains. While preparation of competent cells is a standard procedure in fla...

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Bibliographic Details
Main Authors: Sebastian Hans, Matthias Gimpel, Florian Glauche, Peter Neubauer, Mariano Nicolas Cruz-Bournazou
Format: Article
Language:English
Published: MDPI AG 2018-06-01
Series:Microorganisms
Subjects:
competent cells
Escherichia coli
turbidostat
automation
high throughput
chemostat
transformation
Online Access:http://www.mdpi.com/2076-2607/6/3/60

Internet

http://www.mdpi.com/2076-2607/6/3/60

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