Automated Cell Treatment for Competence and Transformation of Escherichia coli in a High-Throughput Quasi-Turbidostat Using Microtiter Plates
Metabolic engineering and genome editing strategies often lead to large strain libraries of a bacterial host. Nevertheless, the generation of competent cells is the basis for transformation and subsequent screening of these strains. While preparation of competent cells is a standard procedure in fla...
Main Authors: | Sebastian Hans, Matthias Gimpel, Florian Glauche, Peter Neubauer, Mariano Nicolas Cruz-Bournazou |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-06-01
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Series: | Microorganisms |
Subjects: | |
Online Access: | http://www.mdpi.com/2076-2607/6/3/60 |
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