A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae

A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae

Summary: Characterizing mRNA and protein expression with temporal and spatial resolution is a valuable component of nearly every developmental study. Here, we describe a protocol that combines in situ hybridization chain reaction (HCR) and immunofluorescence, allowing for the detection of mRNAs and...

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Bibliographic Details
Main Authors: Rodrigo Ibarra-García-Padilla, Aubrey Gaylon Adam Howard, IV, Eileen Willey Singleton, Rosa Anna Uribe
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Developmental biology
Microscopy
Model Organisms
Molecular Biology
Antibody
In Situ Hybridization
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004160

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http://www.sciencedirect.com/science/article/pii/S2666166721004160

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