A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae
Summary: Characterizing mRNA and protein expression with temporal and spatial resolution is a valuable component of nearly every developmental study. Here, we describe a protocol that combines in situ hybridization chain reaction (HCR) and immunofluorescence, allowing for the detection of mRNAs and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721004160 |
| _version_ | 1818915294840619008 |
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| author | Rodrigo Ibarra-García-Padilla Aubrey Gaylon Adam Howard, IV Eileen Willey Singleton Rosa Anna Uribe |
| author_facet | Rodrigo Ibarra-García-Padilla Aubrey Gaylon Adam Howard, IV Eileen Willey Singleton Rosa Anna Uribe |
| author_sort | Rodrigo Ibarra-García-Padilla |
| collection | DOAJ |
| description | Summary: Characterizing mRNA and protein expression with temporal and spatial resolution is a valuable component of nearly every developmental study. Here, we describe a protocol that combines in situ hybridization chain reaction (HCR) and immunofluorescence, allowing for the detection of mRNAs and proteins simultaneously, in zebrafish embryos and larvae. This protocol expands the flexibility of multiplexed HCR by coupling it with traditional immunofluorescence detection.For complete details on the use and execution of this protocol, please refer to Choi et al. (2010, 2016, 2018) and Howard et al. (2021). |
| first_indexed | 2024-12-20T00:00:00Z |
| format | Article |
| id | doaj.art-9aa71570916147099f4dbf224d261428 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-20T00:00:00Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-9aa71570916147099f4dbf224d2614282022-12-21T20:00:52ZengElsevierSTAR Protocols2666-16672021-09-0123100709A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvaeRodrigo Ibarra-García-Padilla0Aubrey Gaylon Adam Howard, IV1Eileen Willey Singleton2Rosa Anna Uribe3Department of Biosciences, Rice University, Houston, TX 77005, USADepartment of Biosciences, Rice University, Houston, TX 77005, USADepartment of Biosciences, Rice University, Houston, TX 77005, USADepartment of Biosciences, Rice University, Houston, TX 77005, USA; Corresponding authorSummary: Characterizing mRNA and protein expression with temporal and spatial resolution is a valuable component of nearly every developmental study. Here, we describe a protocol that combines in situ hybridization chain reaction (HCR) and immunofluorescence, allowing for the detection of mRNAs and proteins simultaneously, in zebrafish embryos and larvae. This protocol expands the flexibility of multiplexed HCR by coupling it with traditional immunofluorescence detection.For complete details on the use and execution of this protocol, please refer to Choi et al. (2010, 2016, 2018) and Howard et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721004160Developmental biologyMicroscopyModel OrganismsMolecular BiologyAntibodyIn Situ Hybridization |
| spellingShingle | Rodrigo Ibarra-García-Padilla Aubrey Gaylon Adam Howard, IV Eileen Willey Singleton Rosa Anna Uribe A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae STAR Protocols Developmental biology Microscopy Model Organisms Molecular Biology Antibody In Situ Hybridization |
| title | A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae |
| title_full | A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae |
| title_fullStr | A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae |
| title_full_unstemmed | A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae |
| title_short | A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae |
| title_sort | protocol for whole mount immuno coupled hybridization chain reaction wichcr in zebrafish embryos and larvae |
| topic | Developmental biology Microscopy Model Organisms Molecular Biology Antibody In Situ Hybridization |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721004160 |
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