Colour-coded nanoscale calibration and optical quantification of axial fluorophore position

Total internal reflection fluorescence (TIRF) has come of age, but a reliable and easy-to-use tool for calibrating evanescent-wave penetration depths is missing. We provide a test-sample for TIRF and other axial super-resolution microscopies for emitter axial calibration. Our originality is that nan...

Full description

Bibliographic Details
Main Authors: Olevsko-Arad Ilya, Feldberg Moshe, Oheim Martin, Salomon Adi
Format: Article
Language:English
Published: EDP Sciences 2023-01-01
Series:EPJ Web of Conferences
Online Access:https://www.epj-conferences.org/articles/epjconf/pdf/2023/13/epjconf_eosam2023_09034.pdf