Optimization of Extraction Process of Tea Protein by Mixed Reverse Microemulsion System
Three reverse microemulsion systems were prepared using sodium bis (2-ethylhexyl) sulfosuccinate (AOT), cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS) and Tween80, and the effects of surfactant concentration, ionic surfactant content, water content (W0), aqueous phase pH, extrac...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-07-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100122 |
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| author | Yang ZHANG Cheng DAI Ziming TAN Lu LI Yan LI Xin'an XIE |
| author_facet | Yang ZHANG Cheng DAI Ziming TAN Lu LI Yan LI Xin'an XIE |
| author_sort | Yang ZHANG |
| collection | DOAJ |
| description | Three reverse microemulsion systems were prepared using sodium bis (2-ethylhexyl) sulfosuccinate (AOT), cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS) and Tween80, and the effects of surfactant concentration, ionic surfactant content, water content (W0), aqueous phase pH, extraction temperature and extraction time on protein forward extraction yield and KCl concentration, aqueous phase pH and extraction temperature on protein backward extraction yield were investigated by single-factor experiments, then the optimal extraction conditions were obtained by orthogonal experiments. The results showed that the Tween80-CTAB microemulsion system was effective for the extraction of tea residues protein, and under the optimal conditions of surfactant concentration of 0.10 mol/L, ionic surfactant content of 70%, aqueous phase pH13.0, W0 25, extraction temperature of 40 ℃ and extraction time of 40 min, the forward extraction yield of tea residues protein reached the maximum value of 16.17%. Under the optimal conditions of KCl concentration of 1.2 mol/L, pH7.0 and extraction temperature of 40 ℃, the backward extraction yield of residues protein could reach 94.78%. SDS-PAGE showed that the molecular bands of tea residues protein obtained by reverse microemulsion extraction were small, which could selectively extract small molecular proteins. |
| first_indexed | 2024-04-12T08:48:34Z |
| format | Article |
| id | doaj.art-9ac1218c6ac94dfda96cca44a7f58d50 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-12T08:48:34Z |
| publishDate | 2022-07-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-9ac1218c6ac94dfda96cca44a7f58d502022-12-22T03:39:38ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-07-01431419420310.13386/j.issn1002-0306.20211001222021100122-14Optimization of Extraction Process of Tea Protein by Mixed Reverse Microemulsion SystemYang ZHANG0Cheng DAI1Ziming TAN2Lu LI3Yan LI4Xin'an XIE5College of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaCollege of Food Science, South China Agricultural University, Guangzhou 510642, ChinaThree reverse microemulsion systems were prepared using sodium bis (2-ethylhexyl) sulfosuccinate (AOT), cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS) and Tween80, and the effects of surfactant concentration, ionic surfactant content, water content (W0), aqueous phase pH, extraction temperature and extraction time on protein forward extraction yield and KCl concentration, aqueous phase pH and extraction temperature on protein backward extraction yield were investigated by single-factor experiments, then the optimal extraction conditions were obtained by orthogonal experiments. The results showed that the Tween80-CTAB microemulsion system was effective for the extraction of tea residues protein, and under the optimal conditions of surfactant concentration of 0.10 mol/L, ionic surfactant content of 70%, aqueous phase pH13.0, W0 25, extraction temperature of 40 ℃ and extraction time of 40 min, the forward extraction yield of tea residues protein reached the maximum value of 16.17%. Under the optimal conditions of KCl concentration of 1.2 mol/L, pH7.0 and extraction temperature of 40 ℃, the backward extraction yield of residues protein could reach 94.78%. SDS-PAGE showed that the molecular bands of tea residues protein obtained by reverse microemulsion extraction were small, which could selectively extract small molecular proteins.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100122reverse microemulsiontea residues proteinforward extractionbackward extractionsodium bis (2-ethylhexyl) sulfosuccinatecetyltrimethylammonium bromidesodium dodecyl sulfatetween80 |
| spellingShingle | Yang ZHANG Cheng DAI Ziming TAN Lu LI Yan LI Xin'an XIE Optimization of Extraction Process of Tea Protein by Mixed Reverse Microemulsion System Shipin gongye ke-ji reverse microemulsion tea residues protein forward extraction backward extraction sodium bis (2-ethylhexyl) sulfosuccinate cetyltrimethylammonium bromide sodium dodecyl sulfate tween80 |
| title | Optimization of Extraction Process of Tea Protein by Mixed Reverse Microemulsion System |
| title_full | Optimization of Extraction Process of Tea Protein by Mixed Reverse Microemulsion System |
| title_fullStr | Optimization of Extraction Process of Tea Protein by Mixed Reverse Microemulsion System |
| title_full_unstemmed | Optimization of Extraction Process of Tea Protein by Mixed Reverse Microemulsion System |
| title_short | Optimization of Extraction Process of Tea Protein by Mixed Reverse Microemulsion System |
| title_sort | optimization of extraction process of tea protein by mixed reverse microemulsion system |
| topic | reverse microemulsion tea residues protein forward extraction backward extraction sodium bis (2-ethylhexyl) sulfosuccinate cetyltrimethylammonium bromide sodium dodecyl sulfate tween80 |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100122 |
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