High-Level Heterologous Expression of Endo-1,4-β-Xylanase from <i>Penicillium citrinum</i> in <i>Pichia pastoris</i> X-33 Directed through Codon Optimization and Optimized Expression

High-Level Heterologous Expression of Endo-1,4-β-Xylanase from <i>Penicillium citrinum</i> in <i>Pichia pastoris</i> X-33 Directed through Codon Optimization and Optimized Expression

Most common industrial xylanases are produced from filamentous fungi. In this study, the codon-optimized <i>xynA</i> gene encoding xylanase A from the fungus <i>Penicilium citrinum</i> was successfully synthesized and expressed in the yeast <i>Pichia pastoris</i>....

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Bibliographic Details
Main Authors: Chanika Ouephanit, Nassapat Boonvitthya, Sophie Bozonnet, Warawut Chulalaksananukul
Format: Article
Language:English
Published: MDPI AG 2019-09-01
Series:Molecules
Subjects:
codon optimization
overexpression
<i>penicillium citrinum</i>
<i>pichia pastoris</i>
<i>p<sub>gap</sub></i>
<i>p<sub>aox1</sub></i>
xylanase a
Online Access:https://www.mdpi.com/1420-3049/24/19/3515

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https://www.mdpi.com/1420-3049/24/19/3515

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