Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse™ Probe System in challenging sequence environment

Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse™ Probe System in challenging sequence environment

<p>Abstract</p> <p>Probe and primer design for single nucleotide polymorphism (SNP) detection can be very challenging for A-T DNA-rich targets, requiring long sequences with lower specificity and stability, while G-C-rich DNA targets present limited design options to lower GC-conte...

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Bibliographic Details
Main Authors: Belousov Yevgeniy S, Welch Robert A, Sanders Silvia, Mills Alan, Kulchenko Alena, Dempcy Robert, Afonina Irina A, Walburger David K, Glaser Cynthia L, Yadavalli Sunita, Vermeulen Nicolaas MJ, Mahoney Walt
Format: Article
Language:English
Published: BMC 2004-03-01
Series:Human Genomics
Subjects:
MGB Eclipse
modified base
genotyping
SNP
AT-rich
GC-rich
automated
melt curve
Online Access:http://www.humgenomics.com/content/1/3/209

Internet

http://www.humgenomics.com/content/1/3/209

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