Continuous Feeding Reduces the Generation of Metabolic Byproducts and Increases Antibodies Expression in Chinese Hamster Ovary-K1 Cells

Continuous Feeding Reduces the Generation of Metabolic Byproducts and Increases Antibodies Expression in Chinese Hamster Ovary-K1 Cells

Chinese hamster ovary (CHO) cells are the most important host system used for monoclonal antibody (mAb) expression. Moreover, the fed-batch culture mode is the most widely used method to increase mAb expression in CHO cells by increasing the amount of feed. However, a high amount of culture feed res...

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Bibliographic Details
Main Authors: Shang Xiao, Waqas Ahmed, Ali Mohsin, Meijin Guo
Format: Article
Language:English
Published: MDPI AG 2021-09-01
Series:Life
Subjects:
Chinese hamster ovary (CHO)
continuous feeding
lactate accumulation
NH<sub>4</sub><sup>+</sup> metabolism
high mannose content
osmolality
Online Access:https://www.mdpi.com/2075-1729/11/9/945

Internet

https://www.mdpi.com/2075-1729/11/9/945

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