Isolation and Identification of Anthocyanin Component in the Fruits of <i>Acanthopanax Sessiliflorus</i> (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity
<i>Acanthopanax sessiliflorus</i> (Rupr. & Maxim.) Seem. (<i>Araliaceae</i>) is one of the most abundant species of genus <i>Acanthopanax</i>. The fruits of <i>A. sessiliflorus</i> are used in traditional medical protocols as an analgesic, tonic, a...
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/8/1781 |
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| author | Liang Chen Xiulan Xin Hui Feng Shuangshi Li Qiguang Cao Xinying Wang Frank Vriesekoop |
| author_facet | Liang Chen Xiulan Xin Hui Feng Shuangshi Li Qiguang Cao Xinying Wang Frank Vriesekoop |
| author_sort | Liang Chen |
| collection | DOAJ |
| description | <i>Acanthopanax sessiliflorus</i> (Rupr. & Maxim.) Seem. (<i>Araliaceae</i>) is one of the most abundant species of genus <i>Acanthopanax</i>. The fruits of <i>A. sessiliflorus</i> are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor, and immune-stimulating agent. In this work, we carried out a comprehensive investigation into the anthocyanin components in the fruits of <i>A. sessiliflorus</i>. The anthocyanin content in the fresh fruits of <i>A. sessiliflorus</i> was determined by high performance liquid chromatography-diode array detection (HPLC/DAD), and the anthocyanin component was isolated from these using high-speed counter-current chromatography (HSCCC) and elucidated by electro-spray ionization-mass spectrometry (ESI/MS), <sup>1</sup>H- and <sup>13</sup>C-NMR. Its antioxidant activity was evaluated by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). We found that <i>A. sessiliflorus</i> contained a gross anthocyanin content of 121.35 mg/100 g. HSCCC was successfully used for separation and purification of the primary anthocyanin component, cyanidin 3-xylosyl-galactoside. The antioxidant and radical scavenging tests indicated that cyanidin 3-xylosyl-galactoside is a potent antioxidant. |
| first_indexed | 2024-03-10T20:30:14Z |
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| id | doaj.art-9b395e5ff64e4b469389a595822ff1b0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:30:14Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9b395e5ff64e4b469389a595822ff1b02023-11-19T21:29:38ZengMDPI AGMolecules1420-30492020-04-01258178110.3390/molecules25081781Isolation and Identification of Anthocyanin Component in the Fruits of <i>Acanthopanax Sessiliflorus</i> (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant ActivityLiang Chen0Xiulan Xin1Hui Feng2Shuangshi Li3Qiguang Cao4Xinying Wang5Frank Vriesekoop6College of Bioengineering, Beijing Polytechnic, Beijing 100176, ChinaCollege of Bioengineering, Beijing Polytechnic, Beijing 100176, ChinaCollege of Bioengineering, Beijing Polytechnic, Beijing 100176, ChinaCollege of Bioengineering, Beijing Polytechnic, Beijing 100176, ChinaCollege of Bioengineering, Beijing Polytechnic, Beijing 100176, ChinaMSD R&D (China) Ltd. Co., Beijing, 100029, ChinaDepartment of Food Technology and Innovation, Harper Adams University, Newport TF10 8NB, UK<i>Acanthopanax sessiliflorus</i> (Rupr. & Maxim.) Seem. (<i>Araliaceae</i>) is one of the most abundant species of genus <i>Acanthopanax</i>. The fruits of <i>A. sessiliflorus</i> are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor, and immune-stimulating agent. In this work, we carried out a comprehensive investigation into the anthocyanin components in the fruits of <i>A. sessiliflorus</i>. The anthocyanin content in the fresh fruits of <i>A. sessiliflorus</i> was determined by high performance liquid chromatography-diode array detection (HPLC/DAD), and the anthocyanin component was isolated from these using high-speed counter-current chromatography (HSCCC) and elucidated by electro-spray ionization-mass spectrometry (ESI/MS), <sup>1</sup>H- and <sup>13</sup>C-NMR. Its antioxidant activity was evaluated by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). We found that <i>A. sessiliflorus</i> contained a gross anthocyanin content of 121.35 mg/100 g. HSCCC was successfully used for separation and purification of the primary anthocyanin component, cyanidin 3-xylosyl-galactoside. The antioxidant and radical scavenging tests indicated that cyanidin 3-xylosyl-galactoside is a potent antioxidant.https://www.mdpi.com/1420-3049/25/8/1781anthocyanin<i>Acanthopanax sessiliflorus</i> (Rupr. &Maxim.) Seem.high-speed counter-current chromatography (HSCCC)antioxidant activity |
| spellingShingle | Liang Chen Xiulan Xin Hui Feng Shuangshi Li Qiguang Cao Xinying Wang Frank Vriesekoop Isolation and Identification of Anthocyanin Component in the Fruits of <i>Acanthopanax Sessiliflorus</i> (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity Molecules anthocyanin <i>Acanthopanax sessiliflorus</i> (Rupr. & Maxim.) Seem. high-speed counter-current chromatography (HSCCC) antioxidant activity |
| title | Isolation and Identification of Anthocyanin Component in the Fruits of <i>Acanthopanax Sessiliflorus</i> (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity |
| title_full | Isolation and Identification of Anthocyanin Component in the Fruits of <i>Acanthopanax Sessiliflorus</i> (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity |
| title_fullStr | Isolation and Identification of Anthocyanin Component in the Fruits of <i>Acanthopanax Sessiliflorus</i> (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity |
| title_full_unstemmed | Isolation and Identification of Anthocyanin Component in the Fruits of <i>Acanthopanax Sessiliflorus</i> (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity |
| title_short | Isolation and Identification of Anthocyanin Component in the Fruits of <i>Acanthopanax Sessiliflorus</i> (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity |
| title_sort | isolation and identification of anthocyanin component in the fruits of i acanthopanax sessiliflorus i rupr maxim seem by means of high speed counter current chromatography and evaluation of its antioxidant activity |
| topic | anthocyanin <i>Acanthopanax sessiliflorus</i> (Rupr. & Maxim.) Seem. high-speed counter-current chromatography (HSCCC) antioxidant activity |
| url | https://www.mdpi.com/1420-3049/25/8/1781 |
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