Quantifying distortions in two-photon remote focussing images using a volumetric calibration specimen

Quantifying distortions in two-photon remote focussing images using a volumetric calibration specimen

Remote focussing microscopy allows sharp, in-focus images to be acquired at speed from outside of the focal plane of an objective lens without any agitation of the specimen. However, without careful optical alignment, the advantages of remote focussing microscopy could be compromised by the introduc...

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Bibliographic Details
Main Authors: Alexander David Corbett, Rebecca-Ann Beatrice Burton, Gil eBub, Patrick S. Salter, Simon eTuohy, Martin J. Booth, Tony eWilson
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-10-01
Series:Frontiers in Physiology
Subjects:
Cardiac Imaging Techniques
Microscopy, Fluorescence
Optics and Photonics
distortion
remote focussing
Online Access:http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00384/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00384/full

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