A simplified procedure for isolation of primary murine microglia

There are various approaches in which one can isolate microglia from murine brains, such as immunomagnetic, density gradient, FACS and differential adhesive methods. In this procedure a modified flask-tapping approach was used due to its simplicity and reproducibility. Our protocol requires only a s...

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Bibliographic Details
Main Authors: Nathan Scott, Ken Witt, Joseph M Schober
Format: Article
Language:English
Published: Future Science Ltd 2022-12-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/btn-2022-0054