A simplified procedure for isolation of primary murine microglia
There are various approaches in which one can isolate microglia from murine brains, such as immunomagnetic, density gradient, FACS and differential adhesive methods. In this procedure a modified flask-tapping approach was used due to its simplicity and reproducibility. Our protocol requires only a s...
Main Authors: | Nathan Scott, Ken Witt, Joseph M Schober |
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Format: | Article |
Language: | English |
Published: |
Future Science Ltd
2022-12-01
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Series: | BioTechniques |
Subjects: | |
Online Access: | https://www.future-science.com/doi/10.2144/btn-2022-0054 |
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