Utilization of Palm Kernel Cake as a Novel Substrate for Phytase Production by Aspergillus niger USM AI1
The present study was carried out to optimize the cultural conditions of phytase production by Aspergillus niger USM AI1 using palm kernel cake (PKC) as the substrate in a solid state fermentation (SSF) system. The optimized cultural conditions of 10 g of PKC with a particle size of ≤0.5 mm, an inoc...
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| Format: | Article |
| Language: | English |
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North Carolina State University
2014-01-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_1_1488_Ibrahim_Lim_Palm_Kernel_Cake |
| _version_ | 1811256400628678656 |
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| author | Darah Ibrahim Sheh-Hong Lim |
| author_facet | Darah Ibrahim Sheh-Hong Lim |
| author_sort | Darah Ibrahim |
| collection | DOAJ |
| description | The present study was carried out to optimize the cultural conditions of phytase production by Aspergillus niger USM AI1 using palm kernel cake (PKC) as the substrate in a solid state fermentation (SSF) system. The optimized cultural conditions of 10 g of PKC with a particle size of ≤0.5 mm, an inoculum size of 1x105 spores/ mL, moisture content of 60% (v/w), and a mixing frequency of once every 48 hours produced 4.91±0.17 U/g dried substrate of phytase and 1.14±0.04 mg glucosamine/ g dried substrate of fungal growth. Maximum growth as well as enzyme production was recorded on the 4th day of cultivation at room temperature (30±2 ºC). The findings indicated that phytase production increased 171.3% after optimization (4.91±0.17 U/g dried substrate) compared to before optimization (1.81±0.16 U/g dried substrate). This study showed that PKC is a potential medium for phytase production in SSF. |
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| format | Article |
| id | doaj.art-9b783330a7f24172884eae3c18cfb7ba |
| institution | Directory Open Access Journal |
| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-04-12T17:39:53Z |
| publishDate | 2014-01-01 |
| publisher | North Carolina State University |
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| series | BioResources |
| spelling | doaj.art-9b783330a7f24172884eae3c18cfb7ba2022-12-22T03:22:50ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-01-01911488149710.15376/biores.9.1.1488-1497Utilization of Palm Kernel Cake as a Novel Substrate for Phytase Production by Aspergillus niger USM AI1Darah Ibrahim0Sheh-Hong Lim1Industrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, MalaysiaIndustrial Biotechnology Research Laboratory (IBRL), School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, MalaysiaThe present study was carried out to optimize the cultural conditions of phytase production by Aspergillus niger USM AI1 using palm kernel cake (PKC) as the substrate in a solid state fermentation (SSF) system. The optimized cultural conditions of 10 g of PKC with a particle size of ≤0.5 mm, an inoculum size of 1x105 spores/ mL, moisture content of 60% (v/w), and a mixing frequency of once every 48 hours produced 4.91±0.17 U/g dried substrate of phytase and 1.14±0.04 mg glucosamine/ g dried substrate of fungal growth. Maximum growth as well as enzyme production was recorded on the 4th day of cultivation at room temperature (30±2 ºC). The findings indicated that phytase production increased 171.3% after optimization (4.91±0.17 U/g dried substrate) compared to before optimization (1.81±0.16 U/g dried substrate). This study showed that PKC is a potential medium for phytase production in SSF.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_1_1488_Ibrahim_Lim_Palm_Kernel_CakePhytaseAspergillus nigerSolid state fermentationOptimizationPalm kernel cake |
| spellingShingle | Darah Ibrahim Sheh-Hong Lim Utilization of Palm Kernel Cake as a Novel Substrate for Phytase Production by Aspergillus niger USM AI1 BioResources Phytase Aspergillus niger Solid state fermentation Optimization Palm kernel cake |
| title | Utilization of Palm Kernel Cake as a Novel Substrate for Phytase Production by Aspergillus niger USM AI1 |
| title_full | Utilization of Palm Kernel Cake as a Novel Substrate for Phytase Production by Aspergillus niger USM AI1 |
| title_fullStr | Utilization of Palm Kernel Cake as a Novel Substrate for Phytase Production by Aspergillus niger USM AI1 |
| title_full_unstemmed | Utilization of Palm Kernel Cake as a Novel Substrate for Phytase Production by Aspergillus niger USM AI1 |
| title_short | Utilization of Palm Kernel Cake as a Novel Substrate for Phytase Production by Aspergillus niger USM AI1 |
| title_sort | utilization of palm kernel cake as a novel substrate for phytase production by aspergillus niger usm ai1 |
| topic | Phytase Aspergillus niger Solid state fermentation Optimization Palm kernel cake |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_09_1_1488_Ibrahim_Lim_Palm_Kernel_Cake |
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