A feeder-free culture using autogeneic conditioned medium for undifferentiated growth of human embryonic stem cells: Comparative expression profiles of mRNAs, microRNAs and proteins among different feeders and conditioned media

<p>Abstract</p> <p>Background</p> <p>Human embryonic stem (hES) cell lines were derived from the inner cell mass of human blastocysts, and were cultured on mouse embryonic fibroblast (MEF) feeder to maintain undifferentiated growth, extensive renewal capacity, and pluri...

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Bibliographic Details
Main Authors: Chou Chi-Hsien, Yu Sung-Liang, Singh Sher, Tsai Zong-Yun, Li Steven
Format: Article
Language:English
Published: BMC 2010-10-01
Series:BMC Cell Biology
Online Access:http://www.biomedcentral.com/1471-2121/11/76