Antioxidants prevent particulate matter-induced senescence of lung fibroblasts
Particulate matter (PM) contributes to human diseases, particularly lung disease; however, the molecular mechanism of its action is yet to be determined. Herein, we found that prolonged PM exposure induced the cellular senescence of normal lung fibroblasts via a DNA damage-mediated response. This PM...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023013865 |
| _version_ | 1797851864191991808 |
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| author | Sein Jin Sung-Jin Yoon Na-Young Jung Wang Sik Lee Jinyoung Jeong Young-Jun Park Wantae Kim Doo-Byoung Oh Jinho Seo |
| author_facet | Sein Jin Sung-Jin Yoon Na-Young Jung Wang Sik Lee Jinyoung Jeong Young-Jun Park Wantae Kim Doo-Byoung Oh Jinho Seo |
| author_sort | Sein Jin |
| collection | DOAJ |
| description | Particulate matter (PM) contributes to human diseases, particularly lung disease; however, the molecular mechanism of its action is yet to be determined. Herein, we found that prolonged PM exposure induced the cellular senescence of normal lung fibroblasts via a DNA damage-mediated response. This PM-induced senescence (PM-IS) was only observed in lung fibroblasts but not in A549 lung adenocarcinoma cells. Mechanistic analysis revealed that reactive oxygen species (ROS) activate the DNA damage response signaling axis, increasing p53 phosphorylation, ultimately leading to cellular senescence via an increase in p21 expression without affecting the p16-pRB pathway. A549 cells, instead, were resistant to PM-IS due to the PM-induced ROS production suppression. Water-soluble antioxidants, such as vitamin C and N-Acetyl Cysteine, were found to alleviate PM-IS by suppressing ROS production, implying that antioxidants are a promising therapeutic intervention for PM-mediated lung pathogenesis. |
| first_indexed | 2024-04-09T19:23:35Z |
| format | Article |
| id | doaj.art-9bc55958b13348ceaf42d4ae66e76f02 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-09T19:23:35Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-9bc55958b13348ceaf42d4ae66e76f022023-04-05T08:21:10ZengElsevierHeliyon2405-84402023-03-0193e14179Antioxidants prevent particulate matter-induced senescence of lung fibroblastsSein Jin0Sung-Jin Yoon1Na-Young Jung2Wang Sik Lee3Jinyoung Jeong4Young-Jun Park5Wantae Kim6Doo-Byoung Oh7Jinho Seo8Aging Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, South Korea; Department of Biochemistry, Chungnam National University, Daejeon, 34134, South KoreaEnvironmental Disease Research Center, KRIBB, Daejeon, 34141, South KoreaAging Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, South Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon, 34113, South KoreaEnvironmental Disease Research Center, KRIBB, Daejeon, 34141, South KoreaEnvironmental Disease Research Center, KRIBB, Daejeon, 34141, South Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon, 34113, South KoreaEnvironmental Disease Research Center, KRIBB, Daejeon, 34141, South Korea; Department of Biomolecular Science, KRIBB School of Bioscience, UST, Daejeon, 34113, South KoreaDepartment of Biochemistry, Chungnam National University, Daejeon, 34134, South KoreaAging Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, South Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon, 34113, South Korea; Corresponding author. Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon, 34113, South Korea.Aging Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, South Korea; Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon, 34113, South Korea; Corresponding author. Aging Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, South Korea.Particulate matter (PM) contributes to human diseases, particularly lung disease; however, the molecular mechanism of its action is yet to be determined. Herein, we found that prolonged PM exposure induced the cellular senescence of normal lung fibroblasts via a DNA damage-mediated response. This PM-induced senescence (PM-IS) was only observed in lung fibroblasts but not in A549 lung adenocarcinoma cells. Mechanistic analysis revealed that reactive oxygen species (ROS) activate the DNA damage response signaling axis, increasing p53 phosphorylation, ultimately leading to cellular senescence via an increase in p21 expression without affecting the p16-pRB pathway. A549 cells, instead, were resistant to PM-IS due to the PM-induced ROS production suppression. Water-soluble antioxidants, such as vitamin C and N-Acetyl Cysteine, were found to alleviate PM-IS by suppressing ROS production, implying that antioxidants are a promising therapeutic intervention for PM-mediated lung pathogenesis.http://www.sciencedirect.com/science/article/pii/S2405844023013865Particulate matterCellular senescenceReactive oxygen speciesDNA damage ResponseAntioxidants |
| spellingShingle | Sein Jin Sung-Jin Yoon Na-Young Jung Wang Sik Lee Jinyoung Jeong Young-Jun Park Wantae Kim Doo-Byoung Oh Jinho Seo Antioxidants prevent particulate matter-induced senescence of lung fibroblasts Heliyon Particulate matter Cellular senescence Reactive oxygen species DNA damage Response Antioxidants |
| title | Antioxidants prevent particulate matter-induced senescence of lung fibroblasts |
| title_full | Antioxidants prevent particulate matter-induced senescence of lung fibroblasts |
| title_fullStr | Antioxidants prevent particulate matter-induced senescence of lung fibroblasts |
| title_full_unstemmed | Antioxidants prevent particulate matter-induced senescence of lung fibroblasts |
| title_short | Antioxidants prevent particulate matter-induced senescence of lung fibroblasts |
| title_sort | antioxidants prevent particulate matter induced senescence of lung fibroblasts |
| topic | Particulate matter Cellular senescence Reactive oxygen species DNA damage Response Antioxidants |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023013865 |
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