Production and characterization of cell-penetrating recombinant botulinum neurotoxin type A

Summary: We recently reported that fusion of cell-penetrating peptides (CPPs) to botulinum neurotoxin type A (BoNTA) proteins could improve the efficiency of cellular uptake. Here, we describe steps to produce and evaluate CPP-BoNTA fusion proteins. We present procedures for the expression and purif...

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Bibliographic Details
Main Authors: Xuan Wei, Jiage Hu, Liling Jiang, Lu Li, Jia Liu
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722009066